Validation Data Gallery
Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, mouse lung tissue |
| Positive IF/ICC detected in | HepG2 cells, Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 4 publications below |
| IF | See 3 publications below |
| CoIP | See 1 publications below |
Product Information
13550-1-AP targets SFI1 in WB, IF/ICC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4268 Product name: Recombinant human SFI1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 917-1211 aa of BC033613 Sequence: MAQAHPSLLADLGLAFPDFQSPYKIGDPTEISDPPAAPGQPRGTDPMFLSSNTAHSARKQPRRPHFLLEPAQSQRPQKPQEHGLGMAQPAAPSLTRPFLAEAPTALVPHSPLPGALSSAPGPKQPPTASTGPELLLLPLSSFMPCGAAAPARVSAQRATPRDKPPVPSSLASVPDPHLLLPGDFSATRAGPGLSTAGSLDLEAELEEIQQQLLHYQTTKQNLWSCRRQASSLRRWLELNREEPGPEDQEVEQQVQKELEQVEMQIQLLAEELQAQRQPIGACVARIQALRQALC 相同性解析による交差性が予測される生物種 |
| Full Name | Sfi1 homolog, spindle assembly associated (yeast) |
| Calculated molecular weight | 1211 aa, 134 kDa |
| Observed molecular weight | 148 kDa |
| GenBank accession number | BC033613 |
| Gene Symbol | SFI1 |
| Gene ID (NCBI) | 9814 |
| RRID | AB_10596938 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A8K8P3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
human SFI1 is a centriolar protein that associates with a pool of Centrin at the distal end of the centriole. SFI1 forms an interdependent complex with centrin at the centriolar distal end. Human SFI1 is an evolutionarily conserved protein of 1,242 amino acids that contains five residues within full Cdk consensus motifs (S/T*-P-x-K/R), four at the C terminus known to be phosphorylated in vivo (T816, S855, S882, S892) and one immediately preceding the C terminus (PMID: 25340401, 31197030).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SFI1 antibody 13550-1-AP | Download protocol |
| WB protocol for SFI1 antibody 13550-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EBioMedicine PARP inhibitors suppress tumours via centrosome error-induced senescence independent of DNA damage response | ||
J Cell Biol SFI1 promotes centriole duplication by recruiting USP9X to stabilize the microcephaly protein STIL. | ||
J Cell Biol CCDC15 localizes to the centriole inner scaffold and controls centriole length and integrity | ||
Sci Signal Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins. | ||
Sci Rep Dietary glucoraphanin prevents the onset of psychosis in the adult offspring after maternal immune activation. | ||
J Cell Sci Differential requirements for the EF-hands of human centrin2 in primary ciliogenesis and nucleotide excision repair. |