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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated SF3B3 Monoclonal antibody

SF3B3 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

IF

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H7F5

Cat no : CL488-67469

Synonyms

KIAA0017, RSE1, SAP 130, SAP130, SF3b130, SF3B3, Splicing factor 3B subunit 3, STAF130

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL488-67469

    IF Staining of HeLa using CL488-67469

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SF3B3 antibody (CL488-67469, Clone: 2H7F5 ) at dilution of 1:200.

  • IF Staining of HepG2 using CL488-67469

    IF Staining of HepG2 using CL488-67469

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 SF3B3 antibody (CL488-67469, Clone: 2H7F5 ) at dilution of 1:200.

Tested Applications

Positive IF detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy SF3B3 Ready-To-Use IHC Kit. SF3B3 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67469-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL488-67469 targets SF3B3 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SF3B3 fusion protein Ag6315 相同性解析による交差性が予測される生物種
Full Name splicing factor 3b, subunit 3, 130kDa
Calculated molecular weight 136 kDa
Observed molecular weight 130-135 kDa
GenBank accession numberBC003146
Gene symbol SF3B3
Gene ID (NCBI) 23450
RRIDAB_2919480
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SF3B3 antibody CL488-67469Download protocol
Standard Protocols
Click here to view our Standard Protocols