Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, U2OS cells, K-562 cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon cancer tissue, human oesophagus cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
55377-1-AP targets SETD2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | SET domain containing 2 |
| Calculated molecular weight | 288 kDa |
| Observed molecular weight | 287 kDa |
| GenBank accession number | NM_014159 |
| Gene Symbol | SETD2 |
| Gene ID (NCBI) | 29072 |
| RRID | AB_2881318 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYW2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SETD2, also named as HIF1, HYPB, KIAA1732, KMT3A, SET2, p231HBP, hSET2 and HIP-1, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is a histone methyltransferase that methylates 'Lys-36' of histone H3. SETD2 probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. It binds DNA at promoters. SETD2 may also act as a transcription activator that binds to promoters.(PMID:16118227) It is a potential tumor suppressor gene in breast cancer.(PMID:20944102) This antibody is specific to SETD2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SETD2 antibody 55377-1-AP | Download protocol |
| IHC protocol for SETD2 antibody 55377-1-AP | Download protocol |
| WB protocol for SETD2 antibody 55377-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res PHD finger protein 1 (PHF1) is a novel reader for histone H4R3 symmetric dimethylation and coordinates with PRMT5-WDR77/CRL4B complex to promote tumorigenesis.
| ||
Cell Death Dis Knockdown of SETD2 promotes erastin-induced ferroptosis in ccRCC
| ||
Carcinogenesis Proteomic analysis of the OGT interactome: novel links to epithelial-mesenchymal transition and metastasis of cervical cancer. | ||
J Exp Clin Cancer Res A novel FBW7/NFAT1 axis regulates cancer immunity in sunitinib-resistant renal cancer by inducing PD-L1 expression. | ||
Function (Oxf) Structure and Function of RhoBTB1 Required for Substrate Specificity and Cullin-3 Ubiquitination |
