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- KD/KO Validated
SET/TAF-I Polyclonal antibody
SET/TAF-I Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 55201-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells |
| Positive IHC detected in | mouse spleen tissue, human kidney tissue, human liver cancer tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 9 publications below |
| IHC | See 1 publications below |
Product Information
55201-1-AP targets SET/TAF-I in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | SET nuclear oncogene |
| Calculated molecular weight | 33 kDa |
| Observed molecular weight | 41 kDa, 39 kDa |
| GenBank accession number | NM_003011 |
| Gene symbol | SET |
| Gene ID (NCBI) | 6418 |
| RRID | AB_10837360 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SET, also named as IGAAD, I-2PP2A, TAF-I, IGAAD and PHAPII, belongs to the nucleosome assembly protein (NAP) family.SET is a multifunctional oncoprotein which is ubiquitously expressed in all kinds of cells. The SET protein participates in many cellular processes including cell cycle, cell migration, apoptosis, transcription, and DNA repair. The SET protein plays important roles in facilitating cell growth and proliferation via many pathways that promote tumorigenesis and metastasis. The SET protein shows apparent molecular masses of 41 and 39 kDa (PMID: 12759364, PMID: 7753797).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SET/TAF-I antibody 55201-1-AP | Download protocol |
| IHC protocol for SET/TAF-I antibody 55201-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Rep A p53/LINC00324 positive feedback loop suppresses tumor growth by counteracting SET-mediated transcriptional repression
| ||
Oncotarget Overexpression of PP2A inhibitor SET oncoprotein is associated with tumor progression and poor prognosis in human non-small cell lung cancer.
| ||
J Med Chem Selective Peptidomimetic Inhibitors of NTMT1/2: Rational Design, Synthesis, Characterization, and Crystallographic Studies. | ||
Cancers (Basel) Pre-Clinical Study Evaluating Novel Protein Phosphatase 2A Activators as Therapeutics for Neuroblastoma. | ||
BMC Cancer MicroRNA-1915-3p inhibits cell migration and invasion by targeting SET in non-small-cell lung cancer. |
