Validation Data Gallery

Filter: AllWBIPIHCIF-P

WB analysis of C6 using 11303-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 11303-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of rat heart using 11303-1-AP
rat heart tissue were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11303-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 11303-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 11303-1-AP (SERPINE2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.
IP experiment of A549 using 11303-1-AP
IP result of anti-SERPINE2 (IP:11303-1-AP, 4ug; Detection:11303-1-AP 1:10000) with A549 cells lysate 600 ug.
IHC staining of mouse brain using 11303-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11303-1-AP (SERPINE2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11303-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11303-1-AP (SERPINE2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human pancreas cancer using 11303-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 11303-1-AP (SERPINE2 antibody) at dilution of 1:50 (under 10x lens).
IF Staining of human testis using 11303-1-AP
Immunofluorescent analysis of (4% PFA) fixed human testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of human testis using 11303-1-AP
Immunofluorescent analysis of (4% PFA) fixed human testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse testis using 11303-1-AP
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using SERPINE2 antibody (11303-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Featured Product
KD/KO Validated

SERPINE2 Polyclonal antibody

SERPINE2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Cat No. 11303-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Synonyms

PI7, PN1, Serpin E2, GDN, Glia derived nexin

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (21)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, A549 cells, C6 cells, MCF-7 cells, rat heart tissue, mouse kidney tissue, rat brain tissue, rat kidney tissue
Positive IP detected in	A549 cells
Positive IHC detected in	mouse brain tissue, human pancreas cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human testis tissue, mouse testis tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 11 publications below
IHC	See 6 publications below
IF	See 7 publications below
IP	See 3 publications below
CoIP	See 1 publications below

Product Information

11303-1-AP targets SERPINE2 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1830 Product name: Recombinant human SERPINE2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 28-397 aa of BC015663 Sequence: EELGSNTGIQVFNQIVKSRPHDNIVISPHGIASVLGMLQLGADGRTKKQLAMVMRYGVNGVGKILKKINKAIVSKKNKDIVTVANAVFVKNASEIEVPFVTRNKDVFQCEVRNVNFEDPASACDSINAWVKNETRDMIDNLLSPDLIDGVLTRLVLVNAVYFKGLWKSRFQPENTKKRTFVAADGKSYQVPMLAQLSVFRCGSTSAPNDLWYNFIELPYHGESISMLIALPTESSTPLSAIIPHISTKTIDSWMSIMVPKRVQVILPKFTAVAQTDLKEPLKVLGITDMFDSSKANFAKITTGSENLHVSHILQKAKIEVSEDGTKASAATTAILIARSSPPWFIVDRPFLFFIRHNPTGAVLFMGQINK 相同性解析による交差性が予測される生物種
Full Name	serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
Calculated molecular weight	44 kDa
Observed molecular weight	44-50 kDa
GenBank accession number	BC015663
Gene Symbol	SERPINE2
Gene ID (NCBI)	5270
RRID	AB_10732939
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P07093
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.

Protocols

Product Specific Protocols
IF protocol for SERPINE2 antibody 11303-1-AP	Download protocol
IHC protocol for SERPINE2 antibody 11303-1-AP	Download protocol
IP protocol for SERPINE2 antibody 11303-1-AP	Download protocol
WB protocol for SERPINE2 antibody 11303-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IP	Autophagy FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation Authors - Wei Cai View Article
		Adv Sci (Weinh) Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia Authors - Tiemei Li View Article
human	WB,IF	Cell Mol Biol Lett Epigenetic modification regulates the ligamentum flavum hypertrophy through miR-335-3p/SERPINE2/β-catenin signaling pathway Authors - Yongzhao Zhao View Article
human	WB,IP,IHC	Cancer Lett SERPINE2/PN-1 regulates the DNA damage response and radioresistance by activating ATM in lung cancer. Authors - Jingjing Zhang KD Validated View Article
human	WB,IHC,IF	Int J Biol Macromol Construction and validation of an osteosarcoma prognostic model reveals that SERPINE2 contributes to the progression and cisplatin sensitivity in osteosarcoma. Authors - Zhenyi Chen KD Validated View Article
human	IHC	Comput Biol Med Microdissection of cancer-associated fibroblast infiltration subtypes unveils the secreted SERPINE2 contributing to immunosuppressive microenvironment and immuotherapeutic resistance in gastric cancer: A large-scale study integrating bulk and single-cell transcriptome profiling Authors - Dong Zhang View Article

Validation Data Gallery

WB analysis of C6 using 11303-1-AP

WB analysis using 11303-1-AP

WB analysis of rat heart using 11303-1-AP

WB analysis of MCF-7 using 11303-1-AP

WB analysis of A549 using 11303-1-AP

IP experiment of A549 using 11303-1-AP

IHC staining of mouse brain using 11303-1-AP

IHC staining of mouse brain using 11303-1-AP

IHC staining of human pancreas cancer using 11303-1-AP

IF Staining of human testis using 11303-1-AP

IF Staining of human testis using 11303-1-AP

IF Staining of mouse testis using 11303-1-AP

SERPINE2 Polyclonal antibody

SERPINE2 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation

Bone Marrow Mesenchymal Stem Cell-Derived Dermcidin-Containing Migrasomes enhance LC3-Associated Phagocytosis of Pulmonary Macrophages and Protect against Post-Stroke Pneumonia

Epigenetic modification regulates the ligamentum flavum hypertrophy through miR-335-3p/SERPINE2/β-catenin signaling pathway

SERPINE2/PN-1 regulates the DNA damage response and radioresistance by activating ATM in lung cancer.

Construction and validation of an osteosarcoma prognostic model reveals that SERPINE2 contributes to the progression and cisplatin sensitivity in osteosarcoma.

Microdissection of cancer-associated fibroblast infiltration subtypes unveils the secreted SERPINE2 contributing to immunosuppressive microenvironment and immuotherapeutic resistance in gastric cancer: A large-scale study integrating bulk and single-cell transcriptome profiling

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