Validation Data Gallery
Tested Applications
| Positive WB detected in | HepG2 cells, A431 cells, HeLa cells |
| Positive IHC detected in | human bowen disease, human breast cancer tissue, human lung cancer tissue, human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
11722-1-AP targets Maspin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2304 Product name: Recombinant human SERPINB5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-231 aa of BC020713 Sequence: MDALQLANSAFAVDLFKQLCEKEPLGNVLFSPICLSTSLSLAQVGAKGDTANEIGQVLHFENVKDVPFGFQTVTSDVNKLSSFYSLKLIKRLYVDKSLNLSTEFISSTKRPYAKELETVDFKDKLEETKGQINNSIKDLTDGHFENILADNSVNDQTKILVVNAAYFVGKWMKKFPESETKECPFRVNKVCGAACSSKRSPIIDVKNDRDRVGHKSIPMRNLRARPAKCLS 相同性解析による交差性が予測される生物種 |
| Full Name | serpin peptidase inhibitor, clade B (ovalbumin), member 5 |
| Calculated molecular weight | 375 aa, 42 kDa |
| Observed molecular weight | 42 kDa |
| GenBank accession number | BC020713 |
| Gene Symbol | Maspin |
| Gene ID (NCBI) | 5268 |
| RRID | AB_10597111 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P36952 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SERPINB5, also named as PI-5, Maspin and Serpin B5, belongs to the serpin family and Ov-serpin subfamily. It is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, SERPINB5 exhibits no serine protease inhibitory activity.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Maspin antibody 11722-1-AP | Download protocol |
| IHC protocol for Maspin antibody 11722-1-AP | Download protocol |
| WB protocol for Maspin antibody 11722-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Lett Cancer stem-like cell characteristics induced by EB virus-encoded LMP1 contribute to radioresistance in nasopharyngeal carcinoma by suppressing the p53-mediated apoptosis pathway. | ||
Oncol Lett iTRAQ-coupled 2D LC/MS-MS analysis of CXCR7-transfected papillary thyroid carcinoma cells: A new insight into CXCR7 regulation of papillary thyroid carcinoma progression and identification of potential biomarkers. | ||
Cell Death Dis FAT10 promotes chemotherapeutic resistance in pancreatic cancer by inducing epithelial-mesenchymal transition via stabilization of FOXM1 expression. | ||
J Thorac Dis Exploring diverse programmed cell-death patterns to develop a novel gene signature for predicting the prognosis of lung adenocarcinoma patients |
