Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis of human liver using 11851-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 11851-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11851-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 11851-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 11851-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 11851-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11851-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11851-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of RAW264.7 using 11851-1-AP
RAW264.7 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11851-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11851-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11851-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 11851-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 11851-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11851-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 11851-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11851-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 11851-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 11851-1-AP (SERPINA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of L02 using 11851-1-AP
IP result of anti-SERPINA10 (IP:11851-1-AP, 3ug; Detection:11851-1-AP 1:1000) with L02 cells lysate 2500ug.
IHC staining of human liver using 11851-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11851-1-AP (SERPINA10 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 11851-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11851-1-AP (SERPINA10 antibody) at dilution of 1:100 (under 40x lens).
IHC staining of mouse liver using 11851-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11851-1-AP (SERPINA10 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 11851-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11851-1-AP (SERPINA10 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of L02 using 11851-1-AP
Immunofluorescent analysis of L02 cells using 11851-1-AP (SERPINA10 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 11851-1-AP
Immunofluorescent analysis of HepG2 cells, using SERPINA10 antibody 11851-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

SERPINA10 Polyclonal antibody

SERPINA10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11851-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

ZPI, UNQ707/PRO1358, Serpin A10, PZI, PZ-dependent protease inhibitor

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	human liver tissue, HepG2 cells, L02 cells, mouse liver tissue, RAW264.7
Positive IP detected in	L02 cells
Positive IHC detected in	human liver tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	L02 cells, HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11851-1-AP targets SERPINA10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2420 Product name: Recombinant human SERPINA10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 137-444 aa of BC022261 Sequence: KPGLLPSLFKGLRETLSRNLELGLTQGSFAFIHKDFDVKETFFNLSKRYFDTECVPMNFRNASQAKRLMNHYINKETRGKIPKLFDEINPETKLILVDYILFKGKWLTPFDPVFTEVDTFHLDKYKTIKVPMMYGAGKFASTFDKNFRCHVLKLPYQGNATMLVVLMEKMGDHLALEDYLTTDLVETWLRNMKTRNMEVFFPKFKLDQKYEMHELLRQMGIRRIFSPFADLSELSATGRNLQVSRVLQRTVIEVDERGTEAVAGILSEITAYSMPPVIKVDRPFHFMIYEETSGMLLFLGRVVNPTLL 相同性解析による交差性が予測される生物種
Full Name	serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 10
Calculated molecular weight	444 aa, 51 kDa
Observed molecular weight	72 kDa, 68 kDa, 50-55 kDa
GenBank accession number	BC022261
Gene Symbol	SERPINA10
Gene ID (NCBI)	51156
RRID	AB_2254343
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UK55
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for SERPINA10 antibody 11851-1-AP	Download protocol
IHC protocol for SERPINA10 antibody 11851-1-AP	Download protocol
IP protocol for SERPINA10 antibody 11851-1-AP	Download protocol
WB protocol for SERPINA10 antibody 11851-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of human liver using 11851-1-AP

WB analysis of L02 using 11851-1-AP

WB analysis of mouse liver using 11851-1-AP

WB analysis of RAW264.7 using 11851-1-AP

WB analysis of RAW264.7 using 11851-1-AP

WB analysis of L02 using 11851-1-AP

WB analysis of mouse liver using 11851-1-AP

WB analysis of mouse liver using 11851-1-AP

WB analysis of RAW264.7 using 11851-1-AP

WB analysis of mouse liver using 11851-1-AP

WB analysis of HepG2 using 11851-1-AP

WB analysis of HepG2 using 11851-1-AP

WB analysis of L02 using 11851-1-AP

WB analysis of L02 using 11851-1-AP

WB analysis of HepG2 using 11851-1-AP

WB analysis of HepG2 using 11851-1-AP

WB analysis of mouse liver using 11851-1-AP

WB analysis of mouse liver using 11851-1-AP

IP experiment of L02 using 11851-1-AP

IHC staining of human liver using 11851-1-AP

IHC staining of human liver using 11851-1-AP

IHC staining of mouse liver using 11851-1-AP

IHC staining of mouse liver using 11851-1-AP

IF Staining of L02 using 11851-1-AP

IF Staining of HepG2 using 11851-1-AP

SERPINA10 Polyclonal antibody

SERPINA10 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Product Information

Protocols