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Species Reactivity

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  • Featured Product
  • KD/KO Validated

Septin 8 Polyclonal antibody

Septin 8 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11769-1-AP

Synonyms

KIAA0202, SEP2, SEPT8, septin 8, septin8

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 11769-1-AP

    WB analysis of HeLa using 11769-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat brain using 11769-1-AP

    WB analysis of rat brain using 11769-1-AP

    rat brain tissue were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 11769-1-AP

    WB analysis of mouse brain using 11769-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse testis using 11769-1-AP

    WB analysis of mouse testis using 11769-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 11769-1-AP

    WB analysis of HeLa using 11769-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 11769-1-AP

    WB analysis of K-562 using 11769-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 11769-1-AP (Septin 8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 11769-1-AP

    IP experiment of HeLa using 11769-1-AP

    IP result of anti-Septin 8 (IP:11769-1-AP, 3ug; Detection:11769-1-AP 1:1000) with HeLa cells lysate 2000ug.

  • IHC staining of human gliomas using 11769-1-AP

    IHC staining of human gliomas using 11769-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (Septin 8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 11769-1-AP

    IHC staining of human gliomas using 11769-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (SEPT8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 11769-1-AP

    IHC staining of human gliomas using 11769-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (SEPT8 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human heart using 11769-1-AP

    IHC staining of human heart using 11769-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11769-1-AP (Septin 8 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 11769-1-AP

    IHC staining of human heart using 11769-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11769-1-AP (SEPT8 Antibody) at dilution of 1:50 (under 40x lens).

  • IHC staining of human gliomas using 11769-1-AP

    IHC staining of human gliomas using 11769-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (Septin 8 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human gliomas using 11769-1-AP

    IHC staining of human gliomas using 11769-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (Septin 8 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 11769-1-AP

    IHC staining of human gliomas using 11769-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11769-1-AP (SEPT8 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue, K-562 cells, rat brain tissue, mouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11769-1-AP targets Septin 8 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Septin 8 fusion protein Ag2357 相同性解析による交差性が予測される生物種
Full Name septin 8
Calculated molecular weight55.6 kDa
Observed molecular weight 50-56 kDa
GenBank accession numberBC001329
Gene symbol SEPT8
Gene ID (NCBI) 23176
RRIDAB_2185006
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Septin 8, also named as KIAA0202, is a member of the highly conserved septin family. Septins are 40-60kD GTPases that assemble as filamentous scaffolds. They are involved in the organization of submembranous structures, in neuronal polarity, and in vesicle trafficking. It may play a role in platelet secretion. Septin 8 is about 50-56 kDa in WB detection.

Protocols

Product Specific Protocols
WB protocol for Septin 8 antibody 11769-1-APDownload protocol
IHC protocol for Septin 8 antibody 11769-1-APDownload protocol
IP protocol for Septin 8 antibody 11769-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Elife

Septin/anillin filaments scaffold central nervous system myelin to accelerate nerve conduction.

Authors - Julia Patzig
View Article
mouseWB,IF

Thromb Haemost

Impaired Platelet Function in Sept8-Deficient Mice In Vitro

Authors - Katharina Neubauer
View Article

Glia

The formation of paranodal spirals at the ends of CNS myelin sheaths requires the planar polarity protein Vangl2.

Authors - Andrew A Jarjour
View Article
mouseWB

Development

Pinch2 regulates myelination in the mouse central nervous system.

Authors - Joana Paes de Faria
View Article
mouse, humanWB

J Cell Sci

SEPT8 modulates β-amyloidogenic processing of APP by affecting the sorting and accumulation of BACE1.

Authors - Kaisa M A Kurkinen
  • KD Validated
View Article
mouseIF

Cytoskeleton (Hoboken)

Characterization of septin expression in normal and fibrotic kidneys.

Authors - Katharina Neubauer
View Article