- Featured Product
- KD/KO Validated
Septin 6 Polyclonal antibody
Septin 6 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 12805-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells, rat brain tissue |
| Positive IP detected in | rat brain tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 8 publications below |
| IHC | See 1 publications below |
| IF | See 3 publications below |
Product Information
12805-1-AP targets Septin 6 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Septin 6 fusion protein Ag3521 相同性解析による交差性が予測される生物種 |
| Full Name | septin 6 |
| Calculated molecular weight | 434 aa, 50 kDa |
| Observed molecular weight | 50 kDa |
| GenBank accession number | BC036240 |
| Gene symbol | SEPT6 |
| Gene ID (NCBI) | 23157 |
| RRID | AB_2184993 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Septin 6 belongs to the septins family which are conserved GTP-binding proteins functioning as dynamic, regulatable scaffolds for the recruitment of other proteins. They are involved in membrane dynamics, vesicle trafficking, apoptosis, and cytoskeletal remodeling. Septin 6 also interacts with hepatitis C virus (HCV) NS5b RNA polymerase and NS5b-binding protein ( HNRNPA1) and may play a role in HCV RNA replication.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Septin 6 antibody 12805-1-AP | Download protocol |
| IHC protocol for Septin 6 antibody 12805-1-AP | Download protocol |
| IP protocol for Septin 6 antibody 12805-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Lab Invest Effect of SEPT6 on the biological behavior of hepatic stellate cells and liver fibrosis in rats and its mechanism.
| ||
Proteomics Quantification of protein isoforms in mesenchymal stem cells by reductive dimethylation of lysines in intact proteins. | ||
Int J Oncol SEPT6 drives hepatocellular carcinoma cell proliferation, migration and invasion via the Hippo/YAP signaling pathway. | ||
Mol Biol Cell Revised subunit order of mammalian septin complexes explains their in vitro polymerization properties. | ||
Mol Hum Reprod The SEPT12 complex is required for the establishment of a functional sperm head-tail junction. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Mohammed (Verified Customer) (08-19-2020) | Did not show strong staining at the sperm annulus. Overall very high background.
|
