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- KD/KO Validated
SENP5-Specific Polyclonal antibody
SENP5-Specific Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, IHC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 19529-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse liver tissue, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervical cancer tissue, human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:600 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 9 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
19529-1-AP targets SENP5-Specific in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | SUMO1/sentrin specific peptidase 5 |
| Calculated molecular weight | 87 kDa |
| Observed molecular weight | 97 kDa |
| GenBank accession number | NM_152699 |
| Gene symbol | SENP5 |
| Gene ID (NCBI) | 205564 |
| RRID | AB_10643377 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SENP5, also named as FKSG45, belongs to the peptidase C48 family. SENP5 is a protease that catalyzes two essential functions in the SUMO pathway: processing of full-length SUMO3 to its mature form and deconjugation of SUMO2 and SUMO3 from targeted proteins. SENP5 has weak proteolytic activity against full-length SUMO1 or SUMO1 conjugates. It is required for cell division. The antibody is specific to SENP5.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SENP5-Specific antibody 19529-1-AP | Download protocol |
| IHC protocol for SENP5-Specific antibody 19529-1-AP | Download protocol |
| IP protocol for SENP5-Specific antibody 19529-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Cell Sci SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A. | ||
J Cell Sci SUMO proteases SENP3 and SENP5 spatiotemporally regulate the kinase activity of Aurora A. | ||
iScience Drp1 SUMO/deSUMOylation by Senp5 isoforms influences ER tubulation and mitochondrial dynamics to regulate brain development.
| ||
J Comp Neurol Synaptic localization of the SUMOylation-regulating protease SENP5 in the adult mouse brain.
| ||
Cell Rep SENP7 deSUMOylase-governed transcriptional program coordinates sarcomere assembly and is targeted in muscle atrophy |
