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- KD/KO Validated
SECISBP2 Polyclonal antibody
SECISBP2 Polyclonal Antibody for WB, IP, IF, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12798-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, human kidney tissue, Jurkat cells |
| Positive IP detected in | mouse testis tissue |
| Positive IF detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 6 publications below |
| WB | See 14 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
Product Information
12798-1-AP targets SECISBP2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SECISBP2 fusion protein Ag3541 相同性解析による交差性が予測される生物種 |
| Full Name | SECIS binding protein 2 |
| Calculated molecular weight | 854 aa, 95 kDa |
| Observed molecular weight | 95 kDa |
| GenBank accession number | BC036109 |
| Gene symbol | SECISBP2 |
| Gene ID (NCBI) | 79048 |
| RRID | AB_2186555 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Selenium (Se) is an essential trace element required for the biosynthesis of selenoproteins, and selenocysteine insertion sequence (SECIS) binding protein 2 (SECISBP2, or SBP2) represents a key trans-acting factor for the cotranslational insertion of selenocysteine into selenoproteins. Defects in SBP2 are a cause of abnormal thyroid hormone metabolism (ATHYHM) associated with a reduction in type II iodothyronine deiodinase activity. Mutations in this gene have been associated with a reduction in activity of a specific thyroxine deiodinase, a selenocysteine-containing enzyme, and abnormal thyroid hormone metabolism. Cells depleted of SBP2 have increased levels of ROS, which lead to cellular oxidative stress manifested as DNA lesions, stress granules, and lipid peroxidation, induction of caspase- and cytochrome c-dependent apoptosis, indicating that SBP2 is required for protection against ROS-induced cellular damage and cell survival.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SECISBP2 antibody 12798-1-AP | Download protocol |
| IF protocol for SECISBP2 antibody 12798-1-AP | Download protocol |
| IP protocol for SECISBP2 antibody 12798-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science SELENOPROTEINS. CRL2 aids elimination of truncated selenoproteins produced by failed UGA/Sec decoding. | ||
Nat Chem Biol Ribosome stalling during selenoprotein translation exposes a ferroptosis vulnerability. | ||
Nucleic Acids Res Identification of a novel endogenous long non-coding RNA that inhibits selenoprotein P translation. | ||
Free Radic Biol Med Selective up-regulation of human selenoproteins in response to oxidative stress. | ||
Endocrinology Thyroid Hormone Metabolism Defects in a Mouse Model of SBP2 Deficiency.
| ||
J Biol Chem Interplay between Selenium Levels, Selenoprotein Expression and Replicative Senescence in WI-38 Human Fibroblasts. |
