Validation Data Gallery
Tested Applications
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 9 publications below |
| IHC | See 2 publications below |
| IF | See 9 publications below |
| IP | See 1 publications below |
Product Information
13471-1-AP targets SDCCAG8 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, canine, zebrafish samples.
| Tested Reactivity | human, mouse, rat, canine, zebrafish |
| Cited Reactivity | human, mouse, rat, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4264 Product name: Recombinant human SDCCAG8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-360 aa of BC032454 Sequence: MAKSPENSTLEEILGQYQRSLREHASRSIHQLTCALKEGDVTIGEDAPNLSFSTSVGNEDARTAWPELQQSHAVNQLKDLLRQQADKESEVSPSRRRKMSPLRSLEHEETNMPTMHDLVHTINDQSQYIHHLEAEVKFCKEELSGMKNKIQVVVLENEGLQQQLKSQRQEETLREQTLLDASGNMHNSWITTGEDSGVGETSKRPFSHDNADFGKAASAGEQLELEKLKLTYEEKCEIEESQLKFLRNDLAEYQRTCEDLKEQLKHKEFLLAANTCNRVGGLCLKCAQHEAVLSQTHTNVHMQTIERLVKERDDLMSALVSVRSSLADTQQREASAYEQVKQVLQISEEANFEKTKHPSQ 相同性解析による交差性が予測される生物種 |
| Full Name | serologically defined colon cancer antigen 8 |
| Calculated molecular weight | 713 aa, 83 kDa |
| Observed molecular weight | 83 kDa |
| GenBank accession number | BC032454 |
| Gene Symbol | SDCCAG8 |
| Gene ID (NCBI) | 10806 |
| RRID | AB_2183417 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86SQ7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC. SDCCAG8 has 4 isoforms with MW 83,78,73 and 41 kDa (refer to UniProt). Catalog#13471-1-AP can recognize all the four isoforms.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SDCCAG8 antibody 13471-1-AP | Download protocol |
| IHC protocol for SDCCAG8 antibody 13471-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Genet Candidate exome capture identifies mutation of SDCCAG8 as the cause of a retinal-renal ciliopathy. | ||
Neuron SDCCAG8 Regulates Pericentriolar Material Recruitment and Neuronal Migration in the Developing Cortex. | ||
J Am Soc Nephrol Renal-retinal ciliopathy gene sdccag8 regulates DNA damage response signaling.
| ||
Cell Death Dis Selective loss of RPGRIP1-dependent ciliary targeting of NPHP4, RPGR and SDCCAG8 underlies the degeneration of photoreceptor neurons. |
