• Featured Product
  • KD/KO Validated

SDCCAG3 Polyclonal antibody

SDCCAG3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 15969-1-AP

Synonyms

SDCCAG 3, NY CO 3, ENTR1, Antigen NY-CO-3


☆対象製品が30%オフ!組換えタンパク質&免染用試薬 >>> キャンペーン実施中!


Tested Applications

Positive WB detected inHeLa cells, mouse testis tissue, rat testis tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15969-1-AP targets SDCCAG3 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SDCCAG3 fusion protein Ag8745 相同性解析による交差性が予測される生物種
Full Name serologically defined colon cancer antigen 3
Calculated molecular weight 412 aa, 46 kDa
Observed molecular weight 48-60 kDa
GenBank accession numberBC014515
Gene symbol SDCCAG3
Gene ID (NCBI) 10807
RRIDAB_2183415
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SDCCAG3, also named as NY-CO-3, is identified as serologically defined colon cancer antigen-3. It is an endosomal protein which is involved in the regulation of cytokinesis. SDCCAG3 is involved in modulation of TNF response. This gene has four isoforms with MW 40-50 kDa. Observed MW this protein is 48-60 kDa may due to phosphorylation.

Protocols

Product Specific Protocols
WB protocol for SDCCAG3 antibody 15969-1-APDownload protocol
IHC protocol for SDCCAG3 antibody 15969-1-APDownload protocol
IF protocol for SDCCAG3 antibody 15969-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Commun

Apoptotic signalling targets the post-endocytic sorting machinery of the death receptor Fas/CD95.

Authors - Shruti Sharma
humanWB,IF

Curr Biol

Retromer Binding to FAM21 and the WASH Complex Is Perturbed by the Parkinson Disease-Linked VPS35(D620N) Mutation.

Authors - Ian J McGough
humanWB,IF

Oncogene

The serologically defined colon cancer antigen-3 interacts with the protein tyrosine phosphatase PTPN13 and is involved in the regulation of cytokinesis.

Authors - Hagemann N N
  • KD Validated
humanWB, IF

J Cell Sci

Identification of molecular heterogeneity in SNX27-retromer-mediated endosome-to-plasma membrane recycling.

Authors - Ian J McGough
  • KD Validated
humanWB,IF

Sci Rep

The serologically defined colon cancer antigen-3 (SDCCAG3) is involved in the regulation of ciliogenesis.

Authors - Fangyan Yu
  • KD Validated
ratIF

J Dent Res

Sdccag3 Promotes Implant Osseointegration during Experimental Hyperlipidemia.

Authors - H Ren