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Target

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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

SDC4 Polyclonal antibody

SDC4 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 11820-1-AP

Synonyms

Amphiglycan, Ryudocan core protein, SDC4, SYND4, syndecan 4

Validation Data Gallery

Applications:
  • WB analysis using 11820-1-AP

    WB analysis using 11820-1-AP

    NIH/3T3 lysates were subjected to SDS PAGE followed by western blot with 11820-1-AP (SDC4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse skeletal muscle using 11820-1-AP

    IHC staining of mouse skeletal muscle using 11820-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 11820-1-AP

    IHC staining of mouse skeletal muscle using 11820-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 11820-1-AP

    IHC staining of human liver cancer using 11820-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 11820-1-AP

    IHC staining of human liver cancer using 11820-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 11820-1-AP

    IHC staining of human liver cancer using 11820-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 11820-1-AP

    IHC staining of human liver cancer using 11820-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inNIH/3T3 cells
Positive IHC detected inmouse skeletal muscle tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11820-1-AP targets SDC4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SDC4 fusion protein Ag2388 相同性解析による交差性が予測される生物種
Full Name syndecan 4
Calculated molecular weight 22 kDa
Observed molecular weight 22-26 kDa
GenBank accession numberBC030805
Gene symbol SDC4
Gene ID (NCBI) 6385
RRIDAB_2877797
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SDC4, also named as syndecan 4, is a cell surface proteoglycan that bears heparan sulfate, which is involved in normal wound healing. It has been reported that SDC4may be associated with keratinocyte proliferation and differentiation in the epidermis. SDC4 also plays a key role in the angiogenesis and inflammation. The predicted MW of SDC4 is 22 kDa and 11820-1-AP antibody recognizes a protein around 24-26 kDa and 48-52 kDa (homodimer) which is similar to the papers.(PMID: 20639201, 21949132; 18638267; 30165731 )

Protocols

Product Specific Protocols
WB protocol for SDC4 antibody 11820-1-APDownload protocol
IHC protocol for SDC4 antibody 11820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB,IF

Front Pharmacol

Dioscin Inhibits HSC-T6 Cell Migration via Adjusting SDC-4 Expression: Insights from iTRAQ-Based Quantitative Proteomics.

Authors - Lianhong Yin
View Article
humanWB

Oncol Lett

Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.

Authors - Xiaolin Yang
View Article
humanWB,IF,IHC

Cell Death Dis

Therapeutic potential of targeting membrane-spanning proteoglycan SDC4 in hepatocellular carcinoma.

Authors - Heng Yang
  • KD Validated
View Article
humanWB

eBioMedicine

Atypical E3 ligase ZFP91 promotes small-molecule-induced E2F2 transcription factor degradation for cancer therapy

Authors - Ting-Ting Liu
View Article
mouseIHC,IF

Front Pediatr

Bioinformatics analysis identifies heparan sulfate proteoglycans acting as different progress subtypes of biliary atresia

Authors - Zequan Ding
View Article