Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, A549 cells, SGC-7901 cells, SH-SY5Y cells, U-251 cells, mouse brain tissue |
| Positive IP detected in | SGC-7901 cells |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 8 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
Product Information
11091-1-AP targets EMAP II in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1560 Product name: Recombinant human SCYE1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-312 aa of BC014051 Sequence: MANNDAVLKRLEQKGAEADQIIEYLKQQVSLLKEKAILQATLREEKKLRVENAKLKKEIEELKQELIQAEIQNGVKQIPFPSGTPLHANSMVSENVIQSTAVTTVSSGTKEQIKGGTGDEKKAKEKIEKKGEKKEKKQQSIAGSADSKPIDVSRLDLRIGCIITARKHPDADSLYVEEVDVGEIAPRTVVSGLVNHVPLEQMQNRMVILLCNLKPAKMRGVLSQAMVMCASSPEKIEILAPPNGSVPGDRITFDAFPGEPDKELNPKKKIWEQIQPDLHTNDECVATYKGVPFEVKGKGVCRAQTMSNSGIK 相同性解析による交差性が予測される生物種 |
| Full Name | small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating) |
| Calculated molecular weight | 34 kDa |
| Observed molecular weight | 34 kDa and 68 kDa |
| GenBank accession number | BC014051 |
| Gene Symbol | EMAP II |
| Gene ID (NCBI) | 9255 |
| RRID | AB_2182590 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q12904 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
SCYE1(small inducible cytokine subfamily E, member 1) is also named as EMAP2, AIMP1. The human cDNA encodes a 312-amino acid polypeptide that the predicted 34-kDa precursor can be cleaved to produce an active 20-kDa product(PMID:7929199). SCYE1 is secreted as a result of various stimuli, including TNF-α, heat shock, and hypoxia, and that it acts as a multifunctional cytokine on both endothelial and immune cells(PMID:18292511). Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). It has 2 isoforms produced by alternative splicing and the protein can exsit as a dimer(PMID:11306575).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EMAP II antibody 11091-1-AP | Download protocol |
| IHC protocol for EMAP II antibody 11091-1-AP | Download protocol |
| IP protocol for EMAP II antibody 11091-1-AP | Download protocol |
| WB protocol for EMAP II antibody 11091-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) AIMP1 promotes multiple myeloma malignancy through interacting with ANP32A to mediate histone H3 acetylation | ||
Mol Cell A methyltransferase-independent role for METTL1 in tRNA aminoacylation and oncogenic transformation | ||
Cancer Lett Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis. | ||
Proc Natl Acad Sci U S A Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex
| ||
Int J Mol Med Changes in the expression of endothelial monocyte‑activating polypeptide II in the rat hippocampus following status epilepticus. | ||
iScience Variant-specific interaction of kinectin 1 with the multi-tRNA synthetase complex regulates ER sheet organization. |