Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 23006-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 23006-1-AP (SCP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 23006-1-AP
WB result of SCP2 antibody (23006-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SCP2 transfected HEK-293 cells.
WB analysis of K-562 using 23006-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 23006-1-AP (SCP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of NIH/3T3 using 23006-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23006-1-AP (SCP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 23006-1-AP
IP result of anti-SCP2 (IP:23006-1-AP, 4ug; Detection:23006-1-AP 1:500) with HepG2 cells lysate 3600ug.
IHC staining of human stomach cancer using 23006-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23006-1-AP (SCP2/SCPx antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 23006-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23006-1-AP (SCP2/SCPx antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 23006-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 23006-1-AP (SCP2/SCPx antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse lung using 23006-1-AP
Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 23006-1-AP (SCP2/SCPx antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 23006-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 23006-1-AP (SCP2/SCPx antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse stomach using 23006-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 23006-1-AP (SCP2/SCPx antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 23006-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 23006-1-AP (SCP2/SCPx antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat stomach using 23006-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 23006-1-AP (SCP2/SCPx antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 23006-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23006-1-AP (SCP2/SCPx antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human placenta using 23006-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 23006-1-AP (SCP2/SCPx antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 23006-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 23006-1-AP (SCP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 23006-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human SCP2 (23006-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

SCP2/SCPx Polyclonal antibody

SCP2/SCPx Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 23006-1-AP

Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

SCP2, Acetyl-CoA C-myristoyltransferase, EC:2.3.1.155, EC:2.3.1.16, EC:2.3.1.176

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (19)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293T cells, HEK-293 cells, K-562 cells, NIH/3T3 cells, HepG2 cells
Positive IP detected in	HepG2 cells
Positive IHC detected in	human stomach cancer tissue, human placenta tissue, mouse stomach tissue, rat stomach tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 14 publications below
IHC	See 4 publications below
IF	See 5 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

23006-1-AP targets SCP2/SCPx in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, rabbit
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19215 Product name: Recombinant human SCP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 405-547 aa of BC005911 Sequence: MGFPEAASSFRTHQIEAVPTSSASDGFKANLVFKEIEKKLEEEGEQFVKKIGGIFAFKVKDGPGGKEATWVVDVKNGKGSVLPNSDKKADCTITMADSDFLALMTGKMNPQSAFFQGKLKITGNMGLAMKLQNLQLQPGNAKL 相同性解析による交差性が予測される生物種
Full Name	sterol carrier protein 2
Calculated molecular weight	547 aa, 59 kDa
Observed molecular weight	13-15 kDa
GenBank accession number	BC005911
Gene Symbol	SCP2
Gene ID (NCBI)	6342
RRID	AB_2879197
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P22307
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. 23006-1-AP antibody recognizes the 13-15 kDa SCP2 protein.

Protocols

Product Specific Protocols
FC protocol for SCP2/SCPx antibody 23006-1-AP	Download protocol
IF protocol for SCP2/SCPx antibody 23006-1-AP	Download protocol
IHC protocol for SCP2/SCPx antibody 23006-1-AP	Download protocol
IP protocol for SCP2/SCPx antibody 23006-1-AP	Download protocol
WB protocol for SCP2/SCPx antibody 23006-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IF	Nat Cell Biol YAP maintains the dynamics of TDP-43 condensates and antagonizes TDP-43 pathological aggregates Authors - Jiaqi Zhang View Article
	WB	Nat Cell Biol PEX39 facilitates the peroxisomal import of PTS2-containing proteins Authors - Walter W Chen View Article
mouse	WB	Nat Commun Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis Authors - Yesheng Fu View Article
human	WB	Nat Chem Biol Membrane editing with proximity labeling reveals regulators of lipid homeostasis. Authors - Reika Tei View Article
human	WB,IHC,IF	Adv Sci (Weinh) Erucic Acid, Derived by Lactobacillus Crispatus, Induces Ferroptosis in Cervical Cancer Organoids Through the PPAR-δ Signaling Pathway. Authors - Qianwei Zhen View Article
human	WB,IF,CoIP	Adv Sci (Weinh) TrxR2 Lactylation Facilitates Mitochondrial Protection and Endothelial Ferroptosis Resistance in Diabetic Cardiomyopathy. Authors - Su Li View Article

Validation Data Gallery

WB analysis using 23006-1-AP

WB analysis of HEK-293 using 23006-1-AP

WB analysis of K-562 using 23006-1-AP

WB analysis of NIH/3T3 using 23006-1-AP

IP experiment of HepG2 using 23006-1-AP

IHC staining of human stomach cancer using 23006-1-AP

IHC staining of human stomach cancer using 23006-1-AP

IHC staining of mouse lung using 23006-1-AP

IHC staining of mouse lung using 23006-1-AP

IHC staining of mouse stomach using 23006-1-AP

IHC staining of mouse stomach using 23006-1-AP

IHC staining of rat stomach using 23006-1-AP

IHC staining of rat stomach using 23006-1-AP

IHC staining of human placenta using 23006-1-AP

IHC staining of human placenta using 23006-1-AP

IF Staining of HepG2 using 23006-1-AP

FC experiment of HepG2 using 23006-1-AP

SCP2/SCPx Polyclonal antibody

SCP2/SCPx Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

YAP maintains the dynamics of TDP-43 condensates and antagonizes TDP-43 pathological aggregates

PEX39 facilitates the peroxisomal import of PTS2-containing proteins

Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis

Membrane editing with proximity labeling reveals regulators of lipid homeostasis.

Erucic Acid, Derived by Lactobacillus Crispatus, Induces Ferroptosis in Cervical Cancer Organoids Through the PPAR-δ Signaling Pathway.

TrxR2 Lactylation Facilitates Mitochondrial Protection and Endothelial Ferroptosis Resistance in Diabetic Cardiomyopathy.

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