• Featured Product
  • KD/KO Validated

SCF Polyclonal antibody

SCF Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 26582-1-AP

Synonyms

c Kit ligand, KIT ligand, Kitl, KITLG, KL 1, Mast cell growth factor, MGF, SCF, SF, SHEP7, Stem cell factor



Tested Applications

Positive WB detected inPC-12 cells, NIH/3T3 cells, mouse colon tissue
Positive IHC detected inhuman kidney tissue, human small intestine tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

26582-1-AP targets SCF in WB, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCF fusion protein Ag25075 相同性解析による交差性が予測される生物種
Full Name KIT ligand
Calculated molecular weight 273 aa, 31 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC074725
Gene symbol KITLG
Gene ID (NCBI) 4254
RRIDAB_2880561
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Stem cell factor (SCF) also known as mast cell growth factor, steel factor, and kit ligand, is a hematopoietic growth factor. SCF is a critical protein with key roles in the cell such as hematopoiesis, gametogenesis and melanogenesis. SCF activates multiple signal pathways by binding with c-kit receptor. SCF and its receptor, c-kit ligand (KL), are up-regulated in particular human malignancies including gastrointestinal stromal tumor (GISTs), breast cancer, hematopoietic cell, myeloid leukaemia, and glioma. The SCF/c-KIT system also plays a relevant role in cell fate decision, and its activity is widely known to control cell proliferation, differentiation, survival and apoptosis.

Protocols

Product Specific Protocols
WB protocol for SCF antibody 26582-1-APDownload protocol
IHC protocol for SCF antibody 26582-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int J Mol Sci

Increase in Chymase-Positive Mast Cells in Recurrent Pleomorphic Adenoma and Carcinoma Ex Pleomorphic Adenoma of the Parotid Gland.

Authors - Ichita Kinoshita
humanIHC

Int J Mol Sci

Increased Chymase-Positive Mast Cells in High-Grade Mucoepidermoid Carcinoma of the Parotid Gland

Authors - Hiromi Nishimura
mouseWB,IHC

Am J Physiol Gastrointest Liver Physiol

miR-128 participates in the pathogenesis of chronic constipation by regulating the p38α/M-CSF inflammatory signaling pathway.

Authors - Yuntian Hong
mouseWB,IHC

Exp Ther Med

Arctiin alleviates functional constipation by enhancing intestinal motility in mice

Authors - Yujin Wang
humanWB

Thorac Cancer

High expression of KITLG is a new hallmark activating the MAPK pathway in type A and AB thymoma.

Authors - Zhaoyu Yang
  • KD Validated
mouse,humanWB

Chem Biodivers

Shengxuening Extracted from Silkworm Excrement Mitigates Myelosuppression via SCF-Mediated JAK2/STAT3 Signaling.

Authors - Lu Ding