Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
55390-1-AP targets SCARA5 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | scavenger receptor class A, member 5 (putative) |
| Calculated molecular weight | 54 kDa |
| Observed molecular weight | 43 kDa |
| GenBank accession number | NM_173833 |
| Gene Symbol | SCARA5 |
| Gene ID (NCBI) | 286133 |
| RRID | AB_11182487 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6ZMJ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
Background Information
SCARA5 belongs to the SCARA5 family. It is a ferritin receptor that mediates non-transferrin-dependent delivery of iron. SCARA5 mediates cellular uptake of ferritin-bound iron by stimulating ferritin endocytosis from the cell surface with consequent iron delivery within the cell. Delivery of iron to cells by ferritin is required for the development of specific cell types, suggesting the existence of cell type-specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways. Ferritin mediates iron uptake in capsule cells of the developing kidney. SCARA5 binds preferrentially ferritin light chain (FTL) compared to heavy chain (FTH1). This antibody can recognize isoform 1,2 and 3 of SCARA5.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SCARA5 antibody 55390-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Biomater Appl Prussian blue-modified ferritin nanoparticles for effective tumor chemo-photothermal combination therapy via enhancing reactive oxygen species production. |