Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, human liver tissue, MCF-7 cells, mouse pancreas tissue, mouse liver tissue, rat liver tissue |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human colon tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 10 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
14799-1-AP targets RPS4X in WB, IHC, IF/ICC, IP, CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, xenopus, frog |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6513 Product name: Recombinant human SCAR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-263 aa of BC000472 Sequence: MARGPKKHLKRVAAPKHWMLDKLTGVFAPRPSTGPHKLRECLPLIIFLRNRLKYALTGDEVKKICMQRFIKIDGKVRTDITYPAGFMDVISIDKTGENFRLIYDTKGRFAVHRITPEEAKYKLCKVRKIFVGTKGIPHLVTHDARTIRYPDPLIKVNDTIQIDLETGKITDFIKFDTGNLCMVTGGANLGRIGVITNRERHPGSFDVVHVKDANGNSFATRLSNIFVIGKGNKPWISLPRGKGIRLTIAEERDKRLAAKQSSG 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein S4, X-linked |
| Calculated molecular weight | 263 aa, 30 kDa |
| Observed molecular weight | 30 kDa |
| GenBank accession number | BC000472 |
| Gene Symbol | RPS4X |
| Gene ID (NCBI) | 6191 |
| RRID | AB_2238567 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62701 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RPS4X, a X-linked gene in the human, encodes a subunit of ribosomal protein S4, a component of the 40S subunit and 60S subunit. Ribosomal protein S4 is the only ribosomal protein known to be encoded by more than one gene, namely this gene, RPS4Y2 and the ribosomal protein S4, X-linked (RPS4X). [PMID:8358435]
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPS4X antibody 14799-1-AP | Download protocol |
| IHC protocol for RPS4X antibody 14799-1-AP | Download protocol |
| IP protocol for RPS4X antibody 14799-1-AP | Download protocol |
| WB protocol for RPS4X antibody 14799-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Regnase-1 and Roquin Regulate a Common Element in Inflammatory mRNAs by Spatiotemporally Distinct Mechanisms. | ||
Nat Commun Excess ribosomal protein production unbalances translation in a model of Fragile X Syndrome. | ||
Mol Ther Hitchhiking of Cas9 with nucleus-localized proteins impairs its controllability and leads to efficient genome editing of NLS-free Cas9 | ||
Phytomedicine Ailanthone targets the KMT2A-MEN1 complex to suppress lung metastasis of osteosarcoma | ||
Cell Rep Early developmental deletion of forebrain Ank2 causes seizure-related phenotypes by reshaping the synaptic proteome | ||