Validation Data Gallery

Filter: AllWBIPIHC

WB analysis using 26888-1-AP
Huh7 cell lysates were subjected to SDS PAGE followed by western blot with 26888-1-AP (SCAMP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 26888-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 26888-1-AP (SCAMP3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 26888-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 26888-1-AP (SCAMP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 26888-1-AP
IP result of anti-SCAMP3 (IP:26888-1-AP, 4ug; Detection:26888-1-AP 1:5000) with HepG2 cells lysate 1480 ug.
IP experiment of mouse heart using 26888-1-AP
IP result of anti-SCAMP3 (IP:26888-1-AP, 4ug; Detection:26888-1-AP 1:5000) with mouse heart tissue lysate 1880 ug.
IHC staining of mouse skeletal muscle using 26888-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26888-1-AP (SCAMP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse skeletal muscle using 26888-1-AP
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 26888-1-AP (SCAMP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 26888-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26888-1-AP (SCAMP3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 26888-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 26888-1-AP (SCAMP3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

SCAMP3 Polyclonal antibody

SCAMP3 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 26888-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Synonyms

C1orf3, PROPIN1, Secretory carrier-associated membrane protein 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HuH-7 cells, A549 cells, K-562 cells
Positive IP detected in	HepG2 cells, mouse heart tissue
Positive IHC detected in	mouse skeletal muscle tissue, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:3000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 7 publications below
IHC	See 1 publications below
IF	See 1 publications below

Product Information

26888-1-AP targets SCAMP3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25256 Product name: Recombinant human SCAMP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-96 aa of BC000161 Sequence: MAQSRDGGNPFAEPSELDNPFQDPAVIQHRPSRQYATLDVYNPFETREPPPAYEPPAPAPLPPPSAPSLQPSRKLSPTEPKNYGSYSTQASAAAAT 相同性解析による交差性が予測される生物種
Full Name	secretory carrier membrane protein 3
Calculated molecular weight	38 kDa
Observed molecular weight	33 kDa
GenBank accession number	BC000161
Gene Symbol	SCAMP3
Gene ID (NCBI)	10067
RRID	AB_2810962
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O14828
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for SCAMP3 antibody 26888-1-AP	Download protocol
IP protocol for SCAMP3 antibody 26888-1-AP	Download protocol
WB protocol for SCAMP3 antibody 26888-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Mol Cell mTORC1 Activation Requires DRAM-1 by Facilitating Lysosomal Amino Acid Efflux. Authors - Florian Beaumatin KD Validated View Article
human	WB	Aging (Albany NY) Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma. Authors - Feiyu Mao View Article
human	WB	Sci Rep MicroRNA-27a/b-3p and PPARG regulate SCAMP3 through a feed-forward loop during adipogenesis. Authors - Agné Kulyté View Article
human	WB,IHC	Onco Targets Ther SCAMP3 Promotes Glioma Proliferation and Indicates Unfavorable Prognosis via Multiple Pathways. Authors - Chunliu Li KD Validated View Article
human	WB	Onco Targets Ther Bioinformatics Analysis and RNA-Sequencing of SCAMP3 Expression and Correlated Gene Regulation in Hepatocellular Carcinoma. Authors - Shan-Shan Han View Article
human	WB	Phytomedicine Gentianella acuta-derived Gen-miR-1 suppresses myocardial fibrosis by targeting HAX1/HMG20A/Smads axis to attenuate inflammation in cardiac fibroblasts Authors - Tingting Zhang View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 26888-1-AP

WB analysis of A549 using 26888-1-AP

WB analysis of K-562 using 26888-1-AP

IP experiment of HepG2 using 26888-1-AP

IP experiment of mouse heart using 26888-1-AP

IHC staining of mouse skeletal muscle using 26888-1-AP

IHC staining of mouse skeletal muscle using 26888-1-AP

IHC staining of mouse heart using 26888-1-AP

IHC staining of mouse heart using 26888-1-AP

SCAMP3 Polyclonal antibody

SCAMP3 Polyclonal Antibody for WB, IHC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

mTORC1 Activation Requires DRAM-1 by Facilitating Lysosomal Amino Acid Efflux.

Expression and prognostic analyses of SCAMPs in pancreatic adenocarcinoma.

MicroRNA-27a/b-3p and PPARG regulate SCAMP3 through a feed-forward loop during adipogenesis.

SCAMP3 Promotes Glioma Proliferation and Indicates Unfavorable Prognosis via Multiple Pathways.

Bioinformatics Analysis and RNA-Sequencing of SCAMP3 Expression and Correlated Gene Regulation in Hepatocellular Carcinoma.

Gentianella acuta-derived Gen-miR-1 suppresses myocardial fibrosis by targeting HAX1/HMG20A/Smads axis to attenuate inflammation in cardiac fibroblasts