Validation Data Gallery

Filter: AllWB

WB analysis using 21307-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21307-1-AP (SATB2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 21307-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 21307-1-AP (SATB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 21307-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 21307-1-AP (SATB2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Featured Product
KD/KO Validated

SATB2 Polyclonal antibody

SATB2 Polyclonal Antibody for WB, ELISA

Cat No. 21307-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, chip, ELISA

Synonyms

DNA binding protein SATB2, DNA-binding protein SATB2, FLJ21474, FLJ32076, KIAA1034

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in

L02 cells, human brain tissue, mouse liver tissue, rat liver tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 5 publications below
IHC	See 3 publications below
IF	See 1 publications below
ChIP	See 1 publications below

Product Information

21307-1-AP targets SATB2 in WB, IHC, IF, chip, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15920 Product name: Recombinant human SATB2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 228-369 aa of BC098136 Sequence: KYKKIKVERVERENLSDYCVLGQRPMHLPNMNQLASLGKTNEQSPHSQIHHSTPIRNQVPALQPIMSPGLLSPQLSPQLVRQQIAMAHLINQQIAVSRLLAHQHPQAINQQFLNHPPIPRAVKPEPTNSSVEVSPDIYQQVR 相同性解析による交差性が予測される生物種
Full Name	SATB homeobox 2
Calculated molecular weight	733 aa, 83 kDa
Observed molecular weight	80-90 kDa
GenBank accession number	BC098136
Gene Symbol	SATB2
Gene ID (NCBI)	23314
RRID	AB_10733104
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UPW6
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SATB2 antibody 21307-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
rat,mouse	WB,IF	Pharmacol Res Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways. Authors - Jiale Cai View Article
human	chip	Aging (Albany NY) SATB2-Nanog axis links age-related intrinsic changes of mesenchymal stem cells from craniofacial bone. Authors - Peipei Zhou View Article
human	WB	Cell Cycle In vitro experimental study on the formation of microRNA-34a loaded exosomes and their inhibitory effect in oral squamous cell carcinoma. Authors - Wei Deng View Article
rat	WB,IHC	Bone miR-383 negatively regulates osteoblastic differentiation of bone marrow mesenchymal stem cells in rats by targeting Satb2. Authors - Jianfei Tang KD Validated View Article
human	IHC	Cancer Cell Int Identification of key genes involved in tumor immune cell infiltration and cetuximab resistance in colorectal cancer. Authors - Li Liang View Article
human	WB	Mol Carcinog Benzo[a]pyrene diol epoxide-induced transformed cells identify the significance of hsa_circ_0051488, a ERCC1-derived circular RNA in pulmonary squamous cell carcinoma. Authors - Mingyang Xiao View Article

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Validation Data Gallery

WB analysis using 21307-1-AP

WB analysis of human brain using 21307-1-AP

WB analysis of mouse liver using 21307-1-AP

SATB2 Polyclonal antibody

SATB2 Polyclonal Antibody for WB, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Cyclo-(Phe-Tyr) as a novel cyclic dipeptide compound alleviates ischemic/reperfusion brain injury via JUNB/JNK/NF-κB and SOX5/PI3K/AKT pathways.

SATB2-Nanog axis links age-related intrinsic changes of mesenchymal stem cells from craniofacial bone.

In vitro experimental study on the formation of microRNA-34a loaded exosomes and their inhibitory effect in oral squamous cell carcinoma.

miR-383 negatively regulates osteoblastic differentiation of bone marrow mesenchymal stem cells in rats by targeting Satb2.

Identification of key genes involved in tumor immune cell infiltration and cetuximab resistance in colorectal cancer.

Benzo[a]pyrene diol epoxide-induced transformed cells identify the significance of hsa_circ_0051488, a ERCC1-derived circular RNA in pulmonary squamous cell carcinoma.