• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SARS-COV-2 NSP5 Polyclonal antibody

SARS-COV-2 NSP5 Polyclonal Antibody for IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

virus and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 29286-1-AP

Synonyms

SARS-COV-2 NSP5

Validation Data Gallery

Applications:
  • IF Staining of HEK-293T using 29286-1-AP

    IF Staining of HEK-293T using 29286-1-AP

    Immunofluorescent analysis of 4% PFA fixed HEK293T cells overexpressing ACE2 and TMPRSS using NSP5 antibody (29286-1-AP, green) at dilution of 1:200. Cells were infected with virus (dsRNA, in red, MOI 0.01, 48hpi). The images were credited by Dr. Irene Chen, Gladstone Institute.

  • ELISA experiment using 29286-1-AP

    ELISA experiment using 29286-1-AP

    SARS-CoV-2 Envelope protein Antibody (29286-1-AP) tested by ELISA. SARS-COV-2 NSP5 protein was coated onto microtiter plates at 1 µg/well and then incubated with a dilution series of SARS-COV-2 NSP5 protein Antibody (29286-1-AP). Bound antibodies were detected with HRP conjugated anti-Rabbit IgG followed by incubation with HRP Substrate and then measuring the resulting absorbance at 450 nm.

Tested Applications

Positive IF detected inHEK-293T cells
Positive ELISA detected inN/A

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:10-1:100
Enzyme-linked Immunosorbent Assay (ELISA)ELISA :
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

29286-1-AP targets SARS-COV-2 NSP5 in WB, IF, ELISA applications and shows reactivity with virus samples.

Tested Reactivity virus
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SARS-COV-2 NSP5 fusion protein Ag30934 相同性解析による交差性が予測される生物種
Full Name ORF1ab
GenBank accession numberYP_009742612
Gene symbol ORF1ab
Gene ID (NCBI) 43740578
RRIDAB_2918269
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

E3 ubiquitin ligase ZBTB25 suppresses beta coronavirus infection through ubiquitination of the main viral protease MPro

Authors - Travis B Lear
View Article