Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse liver using 15350-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 15350-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 15350-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15350-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15350-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human hepatocirrhosis using 15350-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15350-1-AP (SAR1A antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human hepatocirrhosis using 15350-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15350-1-AP (SAR1A antibody) at dilution of 1:100 (under 40x lens).
IHC staining of human skeletal muscle using 15350-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human skeletal muscle using 15350-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 15350-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 15350-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

SAR1A Polyclonal antibody

SAR1A Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15350-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

COPII associated small GTPase, GTP binding protein SAR1a, masra2, SAR1, SAR1 homolog A (S. cerevisiae), SAR1A, Sara, SARA1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (8)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse liver tissue, HeLa cells, HT-1080 cells
Positive IHC detected in	human hepatocirrhosis tissue, human liver tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 8 publications below
IHC	See 1 publications below
IP	See 1 publications below

Product Information

15350-1-AP targets SAR1A in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7596 Product name: Recombinant human SAR1A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC003658 Sequence: MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYID 相同性解析による交差性が予測される生物種
Full Name	SAR1 homolog A (S. cerevisiae)
Calculated molecular weight	22 kDa
Observed molecular weight	23-25 kDa
GenBank accession number	BC003658
Gene Symbol	SAR1A
Gene ID (NCBI)	56681
RRID	AB_10667406
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NR31
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IHC protocol for SAR1A antibody 15350-1-AP	Download protocol
WB protocol for SAR1A antibody 15350-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Clin Invest Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling. Authors - Zhixiong Liu View Article
human,mouse	WB	Redox Biol Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis Authors - Mengyue You View Article
human	WB	Proc Natl Acad Sci U S A Atlastin-mediated membrane tethering is critical for cargo mobility and exit from the endoplasmic reticulum. Authors - Liling Niu View Article
rat	WB	J Biol Chem The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis. Authors - Lee G D Fryer View Article
human,mouse	IHC,WB	Clin Epigenetics Epigenetic silencing of TMEM176A activates ERK signaling in human hepatocellular carcinoma. Authors - Hongxia Li KD Validated View Article
human	WB,IP	Biochem Biophys Res Commun Pull down assay for GTP-bound form of Sar1a reveals its activation during morphological differentiation. Authors - Yuri Urai View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse liver using 15350-1-AP

WB analysis of HT-1080 using 15350-1-AP

WB analysis of HT-1080 using 15350-1-AP

WB analysis of HeLa using 15350-1-AP

WB analysis of mouse liver using 15350-1-AP

IHC staining of human hepatocirrhosis using 15350-1-AP

IHC staining of human hepatocirrhosis using 15350-1-AP

IHC staining of human skeletal muscle using 15350-1-AP

IHC staining of human skeletal muscle using 15350-1-AP

IHC staining of human liver using 15350-1-AP

IHC staining of human liver using 15350-1-AP

SAR1A Polyclonal antibody

SAR1A Polyclonal Antibody for WB, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.

Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis

Atlastin-mediated membrane tethering is critical for cargo mobility and exit from the endoplasmic reticulum.

The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.

Epigenetic silencing of TMEM176A activates ERK signaling in human hepatocellular carcinoma.

Pull down assay for GTP-bound form of Sar1a reveals its activation during morphological differentiation.