Featured Product
KD/KO Validated

SAR1A Polyclonal antibody

Name: SAR1A Polyclonal antibody
Brand: Proteintech
SKU: 15350-1-AP

SAR1A Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 15350-1-AP

Synonyms

COPII associated small GTPase, GTP binding protein SAR1a, masra2, SAR1, SAR1 homolog A (S. cerevisiae), SAR1A, Sara, SARA1

Validation Data Gallery

Applications:

AllWBIHC

WB analysis of mouse liver using 15350-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 15350-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HT-1080 using 15350-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 15350-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 15350-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 15350-1-AP (SAR1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human hepatocirrhosis using 15350-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15350-1-AP (SAR1A antibody) at dilution of 1:100 (under 10x lens).
Ready-to-use IHC kits available with SAR1A antibody included
IHC staining of human hepatocirrhosis using 15350-1-AP
Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 15350-1-AP (SAR1A antibody) at dilution of 1:100 (under 40x lens).
Ready-to-use IHC kits available with SAR1A antibody included
IHC staining of human liver using 15350-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 10x lens).
Ready-to-use IHC kits available with SAR1A antibody included
IHC staining of human liver using 15350-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 40x lens).
Ready-to-use IHC kits available with SAR1A antibody included
IHC staining of human skeletal muscle using 15350-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 10x lens).
Ready-to-use IHC kits available with SAR1A antibody included
IHC staining of human skeletal muscle using 15350-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 15350-1-AP (SAR1A Antibody) at dilution of 1:50 (under 40x lens).
Ready-to-use IHC kits available with SAR1A antibody included

-/ -

Cat No. 15350-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, HeLa cells, HT-1080 cells
Positive IHC detected in	human hepatocirrhosis tissue, human liver tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Planning an IHC experiment? We recommend our IHCeasy SAR1A Ready-To-Use IHC Kit. SAR1A primary antibody included.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 1 publications below
WB	See 7 publications below
IHC	See 1 publications below
IP	See 1 publications below

Product Information

15350-1-AP targets SAR1A in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	SAR1A fusion protein Ag7596 相同性解析による交差性が予測される生物種
Full Name	SAR1 homolog A (S. cerevisiae)
Calculated molecular weight	22 kDa
Observed molecular weight	23-25 kDa
GenBank accession number	BC003658
Gene symbol	SAR1A
Gene ID (NCBI)	56681
RRID	AB_10667406
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for SAR1A antibody 15350-1-AP	Download protocol
IHC protocol for SAR1A antibody 15350-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Clin Invest Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling. Authors - Zhixiong Liu View Article
human,mouse	WB	Redox Biol Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis Authors - Mengyue You View Article
human	WB	Proc Natl Acad Sci U S A Atlastin-mediated membrane tethering is critical for cargo mobility and exit from the endoplasmic reticulum. Authors - Liling Niu View Article
rat	WB	J Biol Chem The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis. Authors - Lee G D Fryer View Article
human,mouse	IHC,WB	Clin Epigenetics Epigenetic silencing of TMEM176A activates ERK signaling in human hepatocellular carcinoma. Authors - Hongxia Li KD Validated View Article
human	WB,IP	Biochem Biophys Res Commun Pull down assay for GTP-bound form of Sar1a reveals its activation during morphological differentiation. Authors - Yuri Urai View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

SAR1A Polyclonal antibody

SAR1A Polyclonal Antibody for IHC, WB, ELISA

Validation Data Gallery

WB analysis of mouse liver using 15350-1-AP

WB analysis of HT-1080 using 15350-1-AP

WB analysis of HT-1080 using 15350-1-AP

WB analysis of HeLa using 15350-1-AP

WB analysis of mouse liver using 15350-1-AP

IHC staining of human hepatocirrhosis using 15350-1-AP

IHC staining of human hepatocirrhosis using 15350-1-AP

IHC staining of human liver using 15350-1-AP

IHC staining of human liver using 15350-1-AP

IHC staining of human skeletal muscle using 15350-1-AP

IHC staining of human skeletal muscle using 15350-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Sec13 promotes oligodendrocyte differentiation and myelin repair through autocrine pleiotrophin signaling.

Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis

Atlastin-mediated membrane tethering is critical for cargo mobility and exit from the endoplasmic reticulum.

The Endoplasmic Reticulum COPII-Vesicle Transport Machinery Coordinates Cellular Lipid Secretion and Cholesterol Biosynthesis.

Epigenetic silencing of TMEM176A activates ERK signaling in human hepatocellular carcinoma.

Pull down assay for GTP-bound form of Sar1a reveals its activation during morphological differentiation.