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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Ribosomal protein L4 Recombinant antibody

Ribosomal protein L4 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3K6

Cat no : CL488-80959

Synonyms

60S ribosomal protein L1, 60S ribosomal protein L4, ribosomal protein L4, RPL1, RPL4

Validation Data Gallery

Applications:
  • FC experiment of HepG2 using CL488-80959

    FC experiment of HepG2 using CL488-80959

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Ribosomal protein L4 (CL488-80959, Clone:3K6) (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive FC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 80959-1-PBS

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL488-80959 targets Ribosomal protein L4 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Ribosomal protein L4 fusion protein Ag28728 相同性解析による交差性が予測される生物種
Full Name ribosomal protein L4
Calculated molecular weight 48 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC009888
Gene symbol RPL4
Gene ID (NCBI) 6124
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

60S ribosomal protein L4 is encoded by RPL4 (or RPL1) gene. RPL4 is a component of 60S subunit of ribosome, and belongs to the L4E family. By interacting with c-Myb, RPL4 plays a important role in c-myc expression in response to growth factor and nutritional signals. Recently RPL4 was reported to be able to increase effeiciency of viral recoding sequence.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 Ribosomal protein L4 antibody CL488-80959Download protocol
Standard Protocols
Click here to view our Standard Protocols