Rat KIM-1/HAVCR1 ELISA Kit0 Publications

カタログ番号: KE20012

--

(希望販売価格)

容量: 1 Kit (96 assays)

販売および在庫状況はコスモ・バイオ(株)にお問い合わせください。
distributor

Images See all

概要


製品名:
Rat KIM-1/HAVCR1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatants, Urine

アッセイ方法:
Sandwich

感度:
0.4 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Rat

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Rat Serum 116% 105%-126%
cell culture supernatants 95% 82%-105%
rat urine 100% 84%-116%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 497.2 9.0 1.8
2 20 116.3 1.9 1.6
3 20 28..5 0.8 2.9

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 439.3 29.5 6.7
2 24 107.9 9.6 8.9
3 24 26.4 2.6 9.8

製品概要
KE20012 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The KIM-1 ELISA kit is to be used to detect and quantify protein levels of endogenous KIM-1. The assay recognizes rat KIM-1. An antibody specific for KIM-1 has been pre-coated onto the microwells. The KIM-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for rat KIM-1 is added to detect the captured rat KIM-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
CD365, HAVCR1, Kidney injury molecule 1, KIM 1, KIM1, KIM-1, TIM 1, TIM1, TIM-1
背景説明

Kidney injury molecule 1 (KIM-1), also known as Hepatitis A virus cellular receptor 1 (HAVCR1) or T-cell immunoglobulin and mucin domain 1 (TIM-1), is a class I integral membrane glycoprotein, with an ectodomain containing Ig-like domain and a mucin domain. KIM-1 acts as a membrane receptor for hepatitis A virus (HAV). KIM-1 provides a costimulatory signal for T cell activation and inhibits the development of peripheral tolerance. KIM-1 may be involved in the regulation of asthma and allergic diseases. It has been reported that KIM-1 is shed into urine after acute kidney damage and is a marker of renal tubular injury.


Back
to top