Rat ICAM-1/CD54 ELISA Kit0 Publications

カタログ番号: KE20013

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(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Rat ICAM-1/CD54 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma

アッセイ方法:
Sandwich

感度:
3.1 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Rat

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Rat serum 85% 79%-93%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 547.4 13.9 2.5
2 20 124.0 3.3 2.6
3 20 29.0 0.9 3.2

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 546.0 12.5 2.3
2 24 124.4 2.7 2.2
3 24 27.6 0.7 2.4

製品概要
KE20013 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ICAM-1/CD54 ELISA kit is to be used to detect and quantify protein levels of endogenous ICAM-1/CD54 . The assay recognizes rat ICAM-1/CD54 . An antibody specific for ICAM-1/CD54 has been pre-coated onto the microwells. The ICAM-1/CD54 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for ICAM-1/CD54 is added to detect the captured ICAM-1/CD54 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.

別名:
CD54, ICAM, Icam1
背景説明

Intercellular adhesion molecule 1 (ICAM-1, also known as CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin. It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum. ICAM-1 can exist as membrane-bound form (mICAM-1) and soluble form (sICAM-1). The sICAM-1 arises from alternative splicing and proteolysis of mICAM-1 and appears to be associated with leukocyte activation to produce LFA-1.


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