RTN3 Polyclonal antibody
RTN3 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (3)
Applications
WB, IP, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12055-2-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, human brain tissue, HeLa cells, U-87 MG cells, U2OS cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 15 publications below |
| IF | See 5 publications below |
| IP | See 2 publications below |
Product Information
12055-2-AP targets RTN3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, rat, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RTN3 fusion protein Ag2685 相同性解析による交差性が予測される生物種 |
| Full Name | reticulon 3 |
| Calculated molecular weight | 113 kDa |
| Observed molecular weight | 24 kDa, 100-113 kDa |
| GenBank accession number | BC011394 |
| Gene symbol | RTN3 |
| Gene ID (NCBI) | 10313 |
| RRID | AB_2301357 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). Four mammalian reticulons (RTN1-4) exist. Reticulon3 (RTN3) is highly expressed in neurons. RTN3 has been shown to modulate secretory pathway protein trafficking. It is a negative modulator of BACE1 (β-secretase) proteolytic activity. RTN3 may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RTN3 antibody 12055-2-AP | Download protocol |
| IHC protocol for RTN3 antibody 12055-2-AP | Download protocol |
| IP protocol for RTN3 antibody 12055-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Protein Cell Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice | ||
Acta Neuropathol Integrative proteomics highlight presynaptic alterations and c-Jun misactivation as convergent pathomechanisms in ALS | ||
J Pineal Res Melatonin restores endoplasmic reticulum homeostasis to protect injured neurons in a rat model of chronic cervical cord compression | ||
Dev Cell TAOK2 is an ER-localized kinase that catalyzes the dynamic tethering of ER to microtubules. | ||
Acta Neuropathol Commun Liver X receptor-agonist treatment rescues degeneration in a Drosophila model of hereditary spastic paraplegia. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Chun (Verified Customer) (09-07-2020) | This is a very good antibody for immunoblotting.
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