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  • KD/KO Validated

RRM1 Monoclonal antibody

RRM1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, IP, ELISA

Conjugate

Unconjugated

CloneNo.

5H6F3

Cat no : 60073-2-Ig

Synonyms

RIR1, ribonucleotide reductase M1, Ribonucleoside-diphosphate reductase subunit M1, Ribonucleoside-diphosphate reductase large subunit, EC:1.17.4.1


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Tested Applications

Positive WB detected inK-562 cells, HeLa cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue, human urothelial carcinoma tissue, human colon cancer tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue
Positive IF/ICC detected inHepG2 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:3000-1:8000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60073-2-Ig targets RRM1 in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen RRM1 fusion protein Ag0789 相同性解析による交差性が予測される生物種
Full Name ribonucleotide reductase M1
Calculated molecular weight 90 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC006498
Gene symbol RRM1
Gene ID (NCBI) 6240
RRIDAB_10597538
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribonucleoside-diphosphate reductase functions as a heterodimer of a large and a small subunits in deoxyribonucleotide synthesis. RRM1 constitutes to the large subunit (R1) of ribonucleotide reductase, and it can either form heterodimer with small subunit RRM or RRM2B(PMID:16376858). RRM1 provides the precursors necessary for DNA synthesis. RRM1 can not be detected in quiescent cells, while its mRNA and protein are present throughout the cell cycle in cycling cells(PMID:8188248). Researches showed that RRM1 is involved in carcinogenesis, tumor progression, and the resistance of non-small-cell lung cancer (NSCLC) to treatment. Low level expression of RRM1 in NSCLC is associated with poor survival(PMID:17314339).

Protocols

Product Specific Protocols
WB protocol for RRM1 antibody 60073-2-IgDownload protocol
IHC protocol for RRM1 antibody 60073-2-IgDownload protocol
IF protocol for RRM1 antibody 60073-2-IgDownload protocol
IP protocol for RRM1 antibody 60073-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Mol Med

PMID: 31950591

Authors - Sean G Rudd
humanIF

Head Neck

ATM, THMS and RRM1 protein expression in nasopharyngeal carcinomas (NPC) treated with curative intent.

Authors - Jenny Jaeeun Ko
humanWB

J Gastrointest Oncol

Abrogation of ARF6 in promoting erastin-induced ferroptosis and mitigating capecitabine resistance in gastric cancer cells.

Authors - Donghua Geng
humanIHC

Oncol Lett

PMID: 28521448

Authors - Toru Aoyama
humanIHC

Zhongguo Fei Ai Za Zhi

[Prognostic Analysis of ERCC1, RRM1 and p53 Expressions in Postoperative Stage I-II Lung Cancer.].

Authors - Huimin Wang
humanWB,IF

Cell Death Discov

Knockdown of RRM1 in tumor cells promotes radio-/chemotherapy induced ferroptosis by regulating p53 ubiquitination and p21-GPX4 signaling axis.

Authors - Yang Gao
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Juan Pablo (Verified Customer) (11-29-2018)

Works great in WB. I treated CGN with shh (1ug/ml) to induce proliferation and I see a nice increase in RRM1 protein. You get a clean band around 90kDa.For Immuno works good at 1:100 dilution. Tested in P7 cerebellum

  • Applications: Western Blot, Immunohistochemistry,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cerebellar granule culture from P7 pups.
RRM1 Antibody Western Blot,Immunohistochemistry, validation (1:1000 dilution) in Cerebellar granule culture from P7 pups. (Cat no:60073-2-Ig)