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  • KD/KO Validated

RPS26 Polyclonal antibody

RPS26 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, PLA, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14909-1-AP

Synonyms

40S ribosomal protein S26, ribosomal protein S26, RPS26



Tested Applications

Positive WB detected inmouse ovary tissue, mouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman breast cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14909-1-AP targets RPS26 in WB, PLA, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS26 fusion protein Ag6706 相同性解析による交差性が予測される生物種
Full Name ribosomal protein S26
Calculated molecular weight 13 kDa
Observed molecular weight 18-21 kDa
GenBank accession numberBC002604
Gene symbol RPS26
Gene ID (NCBI) 6231
RRIDAB_2180361
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Diamond-Blackfan anemia (DBA) is a congenital red cell aplasia that is usually diagnosed during early infancy, and strikingly, in addition to defects in red cell maturation the disorder is associated with various physical anomalies in about 40% of patients. To date, heterozygous mutations in nine RP genes have been conclusively associated with DBA, and RPS26 is one of them. There are nine mutation found in RPS26 from DBA patients.

Protocols

Product Specific Protocols
WB protocol for RPS26 antibody 14909-1-APDownload protocol
IHC protocol for RPS26 antibody 14909-1-APDownload protocol
IF protocol for RPS26 antibody 14909-1-APDownload protocol
IP protocol for RPS26 antibody 14909-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Nat Commun

Genome-wide analyses of non-syndromic cleft lip with palate identify 14 novel loci and genetic heterogeneity.

Authors - Yanqin Yu
humanWB

Nat Commun

Genome-wide search for exonic variants affecting translational efficiency.

Authors - Li Quan Q
humanIHC, WB

Proc Natl Acad Sci U S A

Local translation in nuclear condensate amyloid bodies.

Authors - Phaedra R Theodoridis
humanWB, PLA

Elife

Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.

Authors - Max Koppers
humanWB,IP

Oncogene

The ribosomal protein S26 regulates p53 activity in response to DNA damage.

Authors - Cui D D
  • KD Validated
humanWB

Int J Mol Sci

Deficiency of the Ribosomal Protein uL5 Leads to Significant Rearrangements of the Transcriptional and Translational Landscapes in Mammalian Cells.

Authors - Elena S Babaylova

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Shinford (Verified Customer) (09-24-2023)

The esophageal cancer cell line is used. The antibody works very stably, and the concentration can be from 1:1000 to 1:2000.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1500
RPS26 Antibody Western Blot validation (1:1500 dilution) in (Cat no:14909-1-AP)