Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse liver tissue, HepG2 cells, rat liver tissue |
| Positive IHC detected in | human liver tissue, human colon Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 14 publications below |
| IHC | See 2 publications below |
| IF | See 5 publications below |
| IP | See 1 publications below |
| ELISA | See 1 publications below |
Product Information
16683-1-AP targets RPS14 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, zebrafish, arabidopsis |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10012 Product name: Recombinant human RPS14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-151 aa of BC006784 Sequence: MAPRKGKEKKEEQVISLGPQVAEGENVFGVCHIFASFNDTFVHVTDLSGKETICRVTGGMKVKADRDESSPYAAMLAAQDVAQRCKELGITALHIKLRATGGNRTKTPGPGAQSALRALARSGMKIGRIEDVTPIPSDSTRRKGGRRGRRL 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein S14 |
| Calculated molecular weight | 16 kDa |
| Observed molecular weight | 16 kDa |
| GenBank accession number | BC006784 |
| Gene Symbol | RPS14 |
| Gene ID (NCBI) | 6208 |
| RRID | AB_2180158 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62263 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes[ PMID 3785212]. The RPS14 gene, encoding a ribosomal protein that belongs to the 40S ribosome, and mapping to the commonly deleted region (CDR) of the 5q- syndrome, shows haploinsufficiency in the CD34+ cells of patients with this disorder[PMID:22430631].
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RPS14 antibody 16683-1-AP | Download protocol |
| WB protocol for RPS14 antibody 16683-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Muscle fiber Myc is dispensable for muscle growth and its forced expression severely perturbs homeostasis | ||
Nat Commun DDX41 resolves G-quadruplexes to maintain erythroid genome integrity and prevent cGAS-mediated cell death | ||
Nat Commun An oocyte meiotic midbody cap is required for developmental competence in mice | ||
Mol Cell Excess intracellular lactate represses mRNA translation and tumor progression through lactate-tRNA charging. | ||
Nucleic Acids Res Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules. |