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- KD/KO Validated
RPS12 Polyclonal antibody
RPS12 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 16490-1-AP
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive WB detected in | NIH/3T3 cells, HeLa cells, MCF-7 cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 2 publications below |
Product Information
16490-1-AP targets RPS12 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, drosophila |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RPS12 fusion protein Ag9615 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein S12 |
| Calculated molecular weight | 132 aa, 15 kDa |
| Observed molecular weight | 15 kDa |
| GenBank accession number | BC017321 |
| Gene symbol | RPS12 |
| Gene ID (NCBI) | 6206 |
| RRID | AB_2146233 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RPS12 (ribosomal protein S12) is a plastid ribosomal protein which is a part of the 30S ribosomal subunit. Together with S4 and S5 plays an important role in translational accuracy.This antibody specifically recognizes the 15kd RPS12 protein.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS12 antibody 16490-1-AP | Download protocol |
| IHC protocol for RPS12 antibody 16490-1-AP | Download protocol |
| IF protocol for RPS12 antibody 16490-1-AP | Download protocol |
| IP protocol for RPS12 antibody 16490-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Cell Progressive Differentiation and Instructive Capacities of Amniotic Fluid and Cerebrospinal Fluid Proteomes following Neural Tube Closure. | ||
Development Recombineering-based dissection of flanking and paralogous Hox gene functions in mouse reproductive tracts. | ||
Sci Rep HumanaFly: high-throughput transgenesis and expression of breast cancer transcripts in Drosophila eye discovers the RPS12-Wingless signaling axis. | ||
Int J Oncol RPS12-specific shRNA inhibits the proliferation, migration of BGC823 gastric cancer cells with S100A4 as a downstream effector.
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