Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human small intestine tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
15461-1-AP targets RPP25 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RPP25 fusion protein Ag7742 相同性解析による交差性が予測される生物種 |
| Full Name | ribonuclease P/MRP 25kDa subunit |
| Calculated molecular weight | 21 kDa |
| Observed molecular weight | 25 kDa |
| GenBank accession number | BC002497 |
| Gene Symbol | RPP25 |
| Gene ID (NCBI) | 54913 |
| RRID | AB_2878142 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BUL9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ribonuclease P protein subunit p25(RPP25) is a component of ribonuclease P, which is a protein complex that generates mature tRNA molecular by cleaving their 5'-ends. It belongs to histone-like Alba family, also a component of RNase MRP. This subunit binds to RNA.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPP25 antibody 15461-1-AP | Download protocol |
| IHC protocol for RPP25 antibody 15461-1-AP | Download protocol |
| IF protocol for RPP25 antibody 15461-1-AP | Download protocol |
| IP protocol for RPP25 antibody 15461-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|