Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse liver tissue, human liver tissue |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
14980-1-AP targets RPL27 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6893 Product name: Recombinant human RPL27 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-136 aa of BC001700 Sequence: MGKFMKPGKVVLVLAGRYSGRKAVIVKNIDDGTSDRPYSHALVAGIDRYPRKVTAAMGKKKIAKRSKIKSFVKVYNYNHLMPTRYSVDIPLDKTVVNKDVFRDPALKRKARREAKVKFEERYKTGKNKWFFQKLRF 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein L27 |
| Calculated molecular weight | 16 kDa |
| Observed molecular weight | 18 kDa |
| GenBank accession number | BC001700 |
| Gene Symbol | RPL27 |
| Gene ID (NCBI) | 6155 |
| RRID | AB_2181742 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P61353 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RPL27, also named as 60S ribosomal protein L27, is a 136 amino acid protein, which contains one KOW domain and belongs to the ribosomal protein L27e family. The function of RPL27 is still non-known. RPL27 may play a role in protein translation. Like most ribosomal proteins, Ribosomal Protein L27 exists as multiple processed pseudogenes that are scattered throughout the genome.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RPL27 antibody 14980-1-AP | Download protocol |
| WB protocol for RPL27 antibody 14980-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Orthop Translat Comparative proteomic analysis identifies differentially expressed proteins and reveals potential mechanisms of traumatic heterotopic ossification progression. | ||
Physiol Rep Aldosterone and angiotensin II induce protein aggregation in renal proximal tubules. | ||
EMBO Rep miR-132 suppresses transcription of ribosomal proteins to promote protective Th1 immunity. | ||
Brain Sci Identification and Validation of Novel Potential Pathogenesis and Biomarkers to Predict the Neurological Outcome after Cardiac Arrest | ||
Nat Struct Mol Biol Nucleolus assembly impairment leads to two-cell transcriptional repression via NPM1-mediated PRC2 recruitment |
