Validation Data Gallery

Filter: AllWBIHCIF/ICC

WB analysis of COLO 320 using 16740-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 16740-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 16740-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 16740-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of SGC-7901 using 16740-1-AP
SGC-7901 cells were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human colon using 16740-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16740-1-AP (RPL15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).
IF Staining of HeLa using 16740-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16740-1-AP (RPL15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

RPL15 Polyclonal antibody

RPL15 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16740-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RPLY10, RPL10, ribosomal protein L15, Large ribosomal subunit protein eL15, EC45

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (5)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	COLO 320 cells, HeLa cells, K-562 cells, mouse brain tissue, SGC-7901 cells
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:200-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 4 publications below
IF	See 1 publications below

Product Information

16740-1-AP targets RPL15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag9948 Product name: Recombinant human RPL15 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-204 aa of BC070328 Sequence: MGAYKYIQELWRKKQSDVMRFLLRVRCWQYRQLSALHRAPRPTRPDKARRLGYKAKQGYVIYRIRVRRGGRKRPVPKGATYGKPVHHGVNQLKFARSLQSVAEERAGRHCGALRVLNSYWVGEDSTYKFFEVILIDPFHKAIRRNPDTQWITKPVHKHREMRGLTSAGRKSRGLGKGHKFHHTIGGSRRAAWRRRNTLQLHRYR 相同性解析による交差性が予測される生物種
Full Name	ribosomal protein L15
Calculated molecular weight	204 aa, 24 kDa
Observed molecular weight	27 kDa
GenBank accession number	BC070328
Gene Symbol	RPL15
Gene ID (NCBI)	6138
RRID	AB_2301193
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P61313
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
IF protocol for RPL15 antibody 16740-1-AP	Download protocol
IHC protocol for RPL15 antibody 16740-1-AP	Download protocol
WB protocol for RPL15 antibody 16740-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Dev Cell Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility. Authors - Yuqing Lei View Article
pig	WB	Front Immunol Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms. Authors - Xu Ye View Article
human	WB	Cell Human hematopoietic stem cell vulnerability to ferroptosis Authors - Jiawei Zhao View Article
human	IF	Cell Rep Senescent cells form nuclear foci that contain the 26S proteasome Authors - Tomohiro Iriki View Article
human	WB	Cell Rep ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells Authors - M Carmen Lafita-Navarro View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of COLO 320 using 16740-1-AP

WB analysis of K-562 using 16740-1-AP

WB analysis of HeLa using 16740-1-AP

WB analysis of mouse brain using 16740-1-AP

WB analysis of SGC-7901 using 16740-1-AP

IHC staining of human colon using 16740-1-AP

IF Staining of HeLa using 16740-1-AP

RPL15 Polyclonal antibody

RPL15 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.

Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.

Human hematopoietic stem cell vulnerability to ferroptosis

Senescent cells form nuclear foci that contain the 26S proteasome

ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells