Validation Data Gallery
Tested Applications
| Positive WB detected in | COLO 320 cells, human brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IF | See 1 publications below |
Product Information
11396-1-AP targets RPH3A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RPH3A fusion protein Ag1956 相同性解析による交差性が予測される生物種 |
| Full Name | rabphilin 3A homolog (mouse) |
| Calculated molecular weight | 690 aa, 76 kDa |
| Observed molecular weight | 76 kDa |
| GenBank accession number | BC017259 |
| Gene Symbol | RPH3A |
| Gene ID (NCBI) | 22895 |
| RRID | AB_2181145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y2J0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RAB3A is a small G protein that is thought to act in exocytosis of neurotransmitters and hormones in synaptic neurotransmission and cell-cell communication. RPH3A (RPH3A), a RAB3A effector, is a neuronal C2 domain tandem containing protein that is involved in the neuronal transmitter process. Huntington's disease (HD) is a hereditary neurodegenerative disorder characterized by progressive motor disturbances, cognitive defects and dementia. Level of rabphilin 3A is substantially decreased in synapses of most brain regions in R6/1 transgenic mouse model of HD suggesting a role of RPH3A in impaired synaptic transmission.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPH3A antibody 11396-1-AP | Download protocol |
| IHC protocol for RPH3A antibody 11396-1-AP | Download protocol |
| IF protocol for RPH3A antibody 11396-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Integrative omics indicate FMRP sequesters mRNA from translation and deadenylation in human neuronal cells | ||
Front Aging Neurosci Detrimental effects of soluble α-synuclein oligomers at excitatory glutamatergic synapses | ||
Sci Rep Multiple Transcriptomic Analyses Explore Potential Synaptic Biomarker Rabphilin-3A for Alzheimer's Disease | ||
Br J Pharmacol Memantine-induced functional rewiring of the glutamate synapse in the striatum of dopamine transporter knockout rats | ||
Pharmacol Res Rabphilin-3A as a novel target to reverse α-synuclein-induced synaptic loss in Parkinson's disease | ||
Elife Rabphilin 3A binds the N-peptide of SNAP-25 to promote SNARE complex assembly in exocytosis |