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  • KD/KO Validated

RPAP2 Polyclonal antibody

RPAP2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 17401-1-AP

Synonyms

C1orf82, RPAP2



Tested Applications

Positive WB detected inDaudi cells, MCF-7 cells, HEK-293 cells, HeLa cells, MOLT-4 cells, THP-1 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman kidney tissue, mouse cerebellum tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

17401-1-AP targets RPAP2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPAP2 fusion protein Ag11374 相同性解析による交差性が予測される生物種
Full Name RNA polymerase II associated protein 2
Calculated molecular weight 612 aa, 70 kDa
Observed molecular weight 70 kDa
GenBank accession numberBC039014
Gene symbol RPAP2
Gene ID (NCBI) 79871
RRIDAB_2180990
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RPAP2 belongs to the RNA Pol II-associated protein (RPAP) family, a set of proteins involved in human Pol II machinery. Recently it has been reported that RPAP2 is required for efficient transcription of snRNA genes by Pol II. siRNA-mediated knockdown of RPAP2 caused defects in snRNA gene expression. (22137580)

Protocols

Product Specific Protocols
WB protocol for RPAP2 antibody 17401-1-APDownload protocol
IHC protocol for RPAP2 antibody 17401-1-APDownload protocol
IF protocol for RPAP2 antibody 17401-1-APDownload protocol
IP protocol for RPAP2 antibody 17401-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Ser7 phosphorylation of the CTD recruits the RPAP2 Ser5 phosphatase to snRNA genes.

Authors - Egloff Sylvain S
humanWB

Mol Cell

Coordination between Two Branches of the Unfolded Protein Response Determines Apoptotic Cell Fate.

Authors - Tsun-Kai Chang
  • KD Validated
humanWB

Nat Struct Mol Biol

RPRD1A and RPRD1B are human RNA polymerase II C-terminal domain scaffolds for Ser5 dephosphorylation.

Authors - Zuyao Ni
  • KD Validated
humanWB

Nucleic Acids Res

Nuclear import of RNA polymerase II is coupled with nucleocytoplasmic shuttling of the RNA polymerase II-associated protein 2.

Authors - Forget Diane D
humanWB

PLoS One

Herpes Simplex Virus 1 (HSV-1) ICP22 Protein Directly Interacts with Cyclin-Dependent Kinase (CDK)9 to Inhibit RNA Polymerase II Transcription Elongation.

Authors - Justyna Zaborowska
humanWB, IF

Histopathology

Abnormal expression of RNA polymerase II associated proteins in muscle of patients with myofibrillar myopathies.

Authors - Valeria Guglielmi