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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RPAP1 Monoclonal antibody

RPAP1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A6H5

Cat no : 68193-1-Ig

Synonyms

DKFZp727M111, FLJ12732, KIAA1403, RPAP1

Validation Data Gallery

Applications:
  • WB analysis using 68193-1-Ig

    WB analysis using 68193-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68193-1-Ig (RPAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 68193-1-Ig

    WB analysis of mouse brain using 68193-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 68193-1-Ig (RPAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inLNCaP cells, mouse brain tissue, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

68193-1-Ig targets RPAP1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RPAP1 fusion protein Ag7856 相同性解析による交差性が予測される生物種
Full Name RNA polymerase II associated protein 1
Calculated molecular weight 153 kDa
Observed molecular weight 153 kDa
GenBank accession numberBC000246
Gene symbol RPAP1
Gene ID (NCBI) 26015
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNA polymerase II-associated protein 1 (RPAP1) encodes a nuclear 153-kDa protein, and forms an interface between the RNA polymerase II (RNAPII) enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. RPAP1 is required for interaction of the RNA polymerase II complex with acetylated histone H3 and the subsequent DNA transcription.

Protocols

Product Specific Protocols
WB protocol for RPAP1 antibody 68193-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols