RPA3 Polyclonal antibody

RPA3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10692-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

REPA3, Replication factor A protein 3, replication protein A3, 14kDa, RF A protein 3, RP A p14, RPA14, RPA3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inK-562 cells, Jurkat cells, A549 cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:750-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

10692-1-AP targets RPA3 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag1065

Product name: Recombinant human RPA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-121 aa of BC005264

Sequence: MVDMMDLPRSRINAGMLAQFIDKPVCFVGRLEKIHPTGKMFILSDGEGKNGTIELMEPLDEEISGIVEVVGRVTAKATILCTSYVQFKEDSHPFDLGLYNEAVKIIHDFPQFYPLGIVQHD

相同性解析による交差性が予測される生物種
Full Name replication protein A3, 14kDa
Calculated molecular weight 13 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC005264
Gene Symbol RPA3
Gene ID (NCBI) 6119
RRIDAB_2269682
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP35244
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPA3 antibody 10692-1-APDownload protocol
IHC protocol for RPA3 antibody 10692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Sci

UMAD1 contributes to ESCRT-III dynamic subunit turnover during cytokinetic abscission

Authors - James Glover
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