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  • KD/KO Validated

RNMT Polyclonal antibody

RNMT Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13743-1-AP

Synonyms

hcm1p, hCMT1, hCMT1c, hMet, KIAA0398, MET, mRNA cap methyltransferase, RG7MT1, RNMT



Tested Applications

Positive WB detected inhuman kidney tissue, HeLa cells, human liver tissue
Positive IP detected inL02 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

13743-1-AP targets RNMT in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNMT fusion protein Ag4682 相同性解析による交差性が予測される生物種
Full Name RNA (guanine-7-) methyltransferase
Calculated molecular weight 476 aa, 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC036798
Gene symbol RNMT
Gene ID (NCBI) 8731
RRIDAB_2181550
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RNMT, also named as mRNA cap guanine-N7 methyltransferase or KIAA0398, is a 476 amino acid protein, which contains one mRNA cap 0 methyltransferase domain and belongs to the class I-like SAM-binding methyltransferase superfamily. mRNA cap 0 methyltransferase family. RNMT localizes in the nucleus and is widely expressed in various tissues. RNMT as a mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs and binds RNA containing 5'-terminal GpppC.

Protocols

Product Specific Protocols
WB protocol for RNMT antibody 13743-1-APDownload protocol
IHC protocol for RNMT antibody 13743-1-APDownload protocol
IF protocol for RNMT antibody 13743-1-APDownload protocol
IP protocol for RNMT antibody 13743-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell

RNA-Methylation-Dependent RNA Processing Controls the Speed of the Circadian Clock.

Authors - Fustin Jean-Michel JM
  • KD Validated
humanWB

Nucleic Acids Res

Inhibition of cytoplasmic cap methylation identifies 5' TOP mRNAs as recapping targets and reveals recapping sites downstream of native 5' ends.

Authors - Daniel Del Valle Morales
humanWB,CoIP

J Mol Biol

Identification and Characterization of the Interaction Between the Methyl-7-Guanosine Cap Maturation Enzyme RNMT and the Cap-Binding Protein eIF4E.

Authors - Michael J Osborne

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Karthik Subramanian (Verified Customer) (07-18-2019)

The antibody initially worked very well for my western blot applications. Subsequently, however, its performance has considerably become poorer and often results in fainter bands with some amount of non-specificity.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: HeLa