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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RNASEH1 Recombinant antibody

RNASEH1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2C12

Cat no : 82771-1-RR

Synonyms

H1RNA, Ribonuclease H type II, ribonuclease H1, RNase H1, RNASEH1, RNH1

Validation Data Gallery

Applications:
  • WB analysis using 82771-1-RR

    WB analysis using 82771-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82771-1-RR (RNASEH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHeLa cells, A549 cells, HEK-293 cells, U2OS cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

Product Information

82771-1-RR targets RNASEH1 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen RNASEH1 fusion protein Ag7990 相同性解析による交差性が予測される生物種
Full Name ribonuclease H1
Calculated molecular weight 32 kDa
Observed molecular weight 32 kDa
GenBank accession numberBC002973
Gene symbol RNASEH1
Gene ID (NCBI) 246243
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribonucleases H are enzymes that cleave the RNA of RNA/DNA hybrids that form during replication and repair and which could lead to DNA instability if they were not processed. Eukaryotic RNases H are larger and more complex than their prokaryotic counterparts [PMID:12667461]. Eukaryotic RNase H1 has acquired a Hybrid Binding Domain that confers processivity and affinity for the substrate. Because of its essential role and its similarities in structure and enzymatic mechanism to the RNase H of HIV reverse transcriptase, RNASEH1 has been implicated in mitochondrial DNA replication during mouse development [PMID:19228196].

Protocols

Product Specific Protocols
WB protocol for RNASEH1 antibody 82771-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols