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Species Reactivity

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  • Featured Product
  • KD/KO Validated

RHEB Polyclonal antibody

RHEB Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 15924-1-AP

Synonyms

GTP binding protein Rheb, Ras homolog enriched in brain, RHEB, RHEB2

Validation Data Gallery

Applications:
  • WB analysis using 15924-1-AP

    WB analysis using 15924-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of fetal human brain using 15924-1-AP

    WB analysis of fetal human brain using 15924-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of human brain using 15924-1-AP

    WB analysis of human brain using 15924-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis of DU 145 using 15924-1-AP

    WB analysis of DU 145 using 15924-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of 3T3-L1 using 15924-1-AP

    WB analysis of 3T3-L1 using 15924-1-AP

    3T3-L1 cells were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 15924-1-AP

    WB analysis of HeLa using 15924-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15924-1-AP (RHEB Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

  • IHC staining of human brain using 15924-1-AP

    IHC staining of human brain using 15924-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15924-1-AP (RHEB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 15924-1-AP

    IHC staining of human brain using 15924-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 15924-1-AP (RHEB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 15924-1-AP

    IHC staining of mouse brain using 15924-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15924-1-AP (RHEB Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of mouse brain using 15924-1-AP

    IHC staining of mouse brain using 15924-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15924-1-AP (RHEB Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of human pancreas cancer using 15924-1-AP

    IF Staining of human pancreas cancer using 15924-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (15924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of human pancreas cancer using 15924-1-AP

    IF Staining of human pancreas cancer using 15924-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human pancreas cancer tissue using RHEB antibody (15924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 15924-1-AP

    IF Staining of HeLa using 15924-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RHEB antibody (15924-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse pancreas using 15924-1-AP

    IF Staining of mouse pancreas using 15924-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using RHEB antibody (15924-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inDU 145 cells, human brain tissue, HeLa cells, 3T3-L1 cells, fetal human brain tissue
Positive IHC detected inhuman brain tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman pancreas cancer tissue, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

15924-1-AP targets RHEB in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RHEB fusion protein Ag8747 相同性解析による交差性が予測される生物種
Full Name Ras homolog enriched in brain
Calculated molecular weight 184 aa, 20 kDa
Observed molecular weight 15-20 kDa
GenBank accession numberBC016155
Gene symbol RHEB
Gene ID (NCBI) 6009
RRIDAB_2285084
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Rheb is a member of the Ras superfamily small GTPases that plays important roles in the regulation of protein synthesis and growth in response to nutrient and growth factors. It is well known to bind and activate mTOR, a serine/threonine kinase which augments protein synthesis. Rheb has GTPase activity and shuttles between a GDP-bound form and a GTP-bound form, and farnesylation of the protein is required for this activity.

Protocols

Product Specific Protocols
WB protocol for RHEB antibody 15924-1-APDownload protocol
IHC protocol for RHEB antibody 15924-1-APDownload protocol
IF protocol for RHEB antibody 15924-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Cell Metab

Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle.

Authors - Noriaki Shimizu
View Article
mouseIHC

PLoS Biol

RHEB/mTOR hyperactivity causes cortical malformations and epileptic seizures through increased axonal connectivity.

Authors - Martina Proietti Onori
View Article
mouseIF

Metabolism

Rheb1 promotes glucose-stimulated insulin secretion in human and mouse β-cells by upregulating GLUT expression.

Authors - Yan Yang
  • KO Validated
View Article
human

Front Cell Dev Biol

PPT1 Reduction Contributes to Erianin-Induced Growth Inhibition in Oral Squamous Carcinoma Cells.

Authors - Qingqiong Luo
View Article
mouseWB

Stem Cell Res Ther

Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis.

Authors - Caixia Jin
View Article
humanWB

Exp Mol Med

A brain somatic RHEB doublet mutation causes focal cortical dysplasia type II.

Authors - Shanshan Zhao
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain