Validation Data Gallery
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Tested Applications
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 5 publications below |
| IHC | See 3 publications below |
| IP | See 1 publications below |
Product Information
23181-1-AP targets RHBDF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RHBDF2 fusion protein Ag19710 相同性解析による交差性が予測される生物種 |
| Full Name | rhomboid 5 homolog 2 (Drosophila) |
| Calculated molecular weight | 856 aa, 97 kDa |
| GenBank accession number | BC016034 |
| Gene Symbol | RHBDF2 |
| Gene ID (NCBI) | 79651 |
| RRID | AB_2879224 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6PJF5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The gene for rhomboid 5 homolog 2 (RHBDF2) is a protein-coding gene that encodes rhombus protease, iRhom2, which participates in important biological processes. RHBDF2 is significantly upregulated in tumors and has pro-tumor effects.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for RHBDF2 antibody 23181-1-AP | Download protocol |
| IF protocol for RHBDF2 antibody 23181-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell Int RHBDF2 gene functions are correlated to facilitated renal clear cell carcinoma progression.
| ||
FEBS Open Bio RHBDF2 is correlated with immune infiltrates in hepatocellular carcinoma and may have potential as a biomarker | ||
Biotechnol Genet Eng Rev The prognostic value of RHBDF2 in Pan-Cancer, and its correlation with cell Adhesion of Hepatocellular Carcinoma | ||
J Virol The Papain-Like Protease of Porcine Reproductive and Respiratory Syndrome Virus Impedes STING Translocation from the Endoplasmic Reticulum to the Golgi Apparatus by Deubiquitinating STIM1 | ||
Vet Microbiol Porcine reproductive and respiratory syndrome virus infection activates ADAM17 to induce inflammatory responses |