Validation Data Gallery

Filter: AllWBIHC

WB analysis of mouse kidney using 20869-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 20869-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 20869-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis using 20869-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 20869-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 20869-1-AP (RHBDD1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 20869-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20869-1-AP (RHBDD1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human testis using 20869-1-AP
Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 20869-1-AP (RHBDD1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

RHBDD1 Polyclonal antibody

RHBDD1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20869-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

HSD 50, RHBDD1, RHBDL4, rhomboid domain containing 1

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information
Publications (4)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse kidney tissue, HEK-293 cells, mouse testis tissue, PC-3 cells
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 4 publications below

Product Information

20869-1-AP targets RHBDD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	RHBDD1 fusion protein Ag14932 相同性解析による交差性が予測される生物種
Full Name	rhomboid domain containing 1
Calculated molecular weight	315 aa, 36 kDa
Observed molecular weight	36 kDa
GenBank accession number	BC027900
Gene Symbol	RHBDD1
Gene ID (NCBI)	84236
RRID	AB_10733756
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TEB9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for RHBDD1 antibody 20869-1-AP	Download protocol
IHC protocol for RHBDD1 antibody 20869-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	J Biol Chem AAA-ATPase valosin-containing protein (VCP) binds the transcription factor SREBP1 and promotes its proteolytic activation by rhomboid protease RHBDL4. Authors - Koji Shibuya View Article
human	WB	Environ Toxicol Suppression of rhomboid domain-containing 1 produces anticancer effects in pancreatic adenocarcinoma through affection of the AKT/GSK-3β/β-catenin pathway. Authors - Jiong Jiang KD Validated View Article
human	WB	Cell Death Dis Long noncoding RNA AFAP1-AS1 promotes tumor progression and invasion by regulating the miR-2110/Sp1 axis in triple-negative breast cancer. Authors - Xiaohui Zhang View Article
human	WB	Genes Cells Mouse transient receptor potential melastatin 2 (TRPM2) isoform 7 attenuates full-length mouse TRPM2 activity through reductions in its expression by targeting it to ER-associated degradation Authors - Shinichiro Yamamoto KD Validated View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of mouse kidney using 20869-1-AP

WB analysis of mouse testis using 20869-1-AP

WB analysis of HEK-293 using 20869-1-AP

WB analysis using 20869-1-AP

WB analysis of PC-3 using 20869-1-AP

IHC staining of human testis using 20869-1-AP