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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RGS14 Monoclonal antibody

RGS14 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C1B2

Cat no : 67394-1-Ig

Synonyms

RGS14

Validation Data Gallery

Applications:
  • WB analysis of mouse brain using 67394-1-Ig

    WB analysis of mouse brain using 67394-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67394-1-Ig

    WB analysis of HeLa using 67394-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 67394-1-Ig

    WB analysis of HEK-293 using 67394-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 67394-1-Ig

    WB analysis of Jurkat using 67394-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 67394-1-Ig

    WB analysis of NIH/3T3 using 67394-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67394-1-Ig (RGS14 antibody) at dilution of 1:13000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 67394-1-Ig

    IHC staining of mouse brain using 67394-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67394-1-Ig (RGS14 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RGS14 antibody included
  • IHC staining of mouse brain using 67394-1-Ig

    IHC staining of mouse brain using 67394-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67394-1-Ig (RGS14 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with RGS14 antibody included
  • IF Staining of HeLa using 67394-1-Ig

    IF Staining of HeLa using 67394-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RGS14 antibody (67394-1-Ig, Clone: 1C1B2 ) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inmouse brain tissue, HeLa cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy RGS14 Ready-To-Use IHC Kit. RGS14 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:15000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:1000-1:4000
Sample-dependent, check data in validation data gallery

Product Information

67394-1-Ig targets RGS14 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RGS14 fusion protein Ag9477 相同性解析による交差性が予測される生物種
Full Name regulator of G-protein signaling 14
Calculated molecular weight 566 aa, 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC014094
Gene symbol RGS14
Gene ID (NCBI) 10636
RRIDAB_2882638
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RGS14, a member of the R12 subfamily of RGS proteins, is highly expressed in the brain and is a natural suppressor of CA2 hippocampal synaptic plasticity and learning and memory. RGS14 was first identified as a complex scaffolding protein with an unconventional domain structure that allows it to interact with various protein binding partners. RGS14 contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP.

Protocols

Product Specific Protocols
WB protocol for RGS14 antibody 67394-1-IgDownload protocol
IHC protocol for RGS14 antibody 67394-1-IgDownload protocol
IF protocol for RGS14 antibody 67394-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols