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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RFX1 Monoclonal antibody

RFX1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G10H6

Cat no : 68057-1-Ig

Synonyms

EF C, Enhancer factor C, Regulatory factor X 1, RFX, RFX1, Transcription factor RFX1

Validation Data Gallery

Applications:
  • WB analysis using 68057-1-Ig

    WB analysis using 68057-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Lamin B1 Monoclonal antibody (66095-1-Ig) as loading control.

  • WB analysis of U2OS using 68057-1-Ig

    WB analysis of U2OS using 68057-1-Ig

    U2OS cells were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 68057-1-Ig

    WB analysis of HeLa using 68057-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of NIH/3T3 using 68057-1-Ig

    WB analysis of NIH/3T3 using 68057-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68057-1-Ig (RFX1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, NIH/3T3 cells, U2OS cells, K-562 cells, A549 cells, MCF-7 cells, LnCaP cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Sample-dependent, check data in validation data gallery

Product Information

68057-1-Ig targets RFX1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen RFX1 fusion protein Ag25442 相同性解析による交差性が予測される生物種
Full Name regulatory factor X, 1 (influences HLA class II expression)
Calculated molecular weight 105 kDa
Observed molecular weight135 kDa
GenBank accession numberBC049826
Gene symbol RFX1
Gene ID (NCBI) 5989
RRIDAB_2918798
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RFX1, also named as MHC class II regulatory factor RFX1, is a 979 amino acid protein which contains 1 RFX-type winged-helix DNA-binding domain and belongs to the RFX family. RFX1 can binds DNA as a homodimer and heterodimerizes with RFX2 and RFX3. RFX1 as a regulatory factor is essential for MHC class II genes expression. RFX1 protein is modified after translation and its molecular weight could be migrate to 130-140 kDa (PMID: 20189986 ).

Protocols

Product Specific Protocols
WB protocol for RFX1 antibody 68057-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols