Validation Data Gallery
Tested Applications
| Positive WB detected in | PC-3 cells, human placenta tissue, MCF-7 cells |
| Positive IHC detected in | human lung tissue, human brain tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 5 publications below |
| WB | See 6 publications below |
| IHC | See 4 publications below |
| IF | See 1 publications below |
Product Information
20156-1-AP targets RDM1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14077 Product name: Recombinant human RDM1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-253 aa of BC038301 Sequence: QHSLFTAFSQFGLLYSVRVFPNAAVAHPGFYAVIKFYSARAAHRAQKACDRKQLFQKSPVKVRLGTRHKAVQHQALALNSSKCQELANYCFGFNGCSKRIIKLQELSDLEERENEDSMVPLPKQSLKFFCALEVVLPSCDCRSPGIGLVEEPMDKVEEGPLSFLMKRKTAQKLAIQKALSDAFQKLLIVVLESGKIAVEYRPSEDIVGVRCEEELHGLIQVPCSPWKQYGQEEEGYLSDFSLEEEEFRLPELD 相同性解析による交差性が予測される生物種 |
| Full Name | RAD52 motif 1 |
| Calculated molecular weight | 284 aa, 32 kDa |
| Observed molecular weight | 32 kDa |
| GenBank accession number | BC038301 |
| Gene Symbol | RDM1 |
| Gene ID (NCBI) | 201299 |
| RRID | AB_10666433 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8NG50 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RDM1, also named as RAD52B, encodes a RNA recognition motif (RRM)-containing protein confered resistance to the antitumor agent cisplatin. The expression of RDM1 is closely related to the cell proliferation and apoptosis of thyroid carcinoma cells and RDM1 may be a potential marker of lung adenocarcinoma. RDM1 has many isoforms (8 kDa, 12-19 kDa and 25-32 kDa) with long or short N-terminus and a great diversity in the exonic composition of C-terminus generated by alternative pre-mRNA splicing and differential usage of two translational start sites. RDM1 protein can be expressed differently in cytoplasm or nuclear/nucleolar in the absence of stress, proteotoxic stress and mild heat shock, suggesting functional differences among the RDM1 proteins. ( PMID: 28939762, 17905820, 28939762, 30069034)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RDM1 antibody 20156-1-AP | Download protocol |
| IHC protocol for RDM1 antibody 20156-1-AP | Download protocol |
| WB protocol for RDM1 antibody 20156-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Oncol Loss of RDM1 enhances hepatocellular carcinoma progression via p53 and Ras/Raf/ERK pathways.
| ||
J Cell Mol Med RDM1 promotes critical processes in breast cancer tumorigenesis.
| ||
Front Cell Dev Biol A Novel Signature Constructed by RNA-Binding Protein Coding Genes to Improve Overall Survival Prediction of Glioma Patients. | ||
Artif Cells Nanomed Biotechnol RDM1 plays an oncogenic role in human ovarian carcinoma cells.
| ||
FEBS Open Bio RDM1 promotes neuroblastoma growth through the RAS-Raf-MEK-ERK pathway.
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