Validation Data Gallery

Filter: AllWBIPIHC

WB analysis of HeLa using 11614-1-AP
WB result of RBM4 antibody (11614-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM4 transfected HeLa cells.
WB analysis of mouse brain using 11614-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 11614-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11614-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11614-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 11614-1-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 11614-1-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 11614-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human kidney using 11614-1-AP
human kidney tissue were subjected to SDS PAGE followed by western blot with 11614-1-AP (RBM4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse heart using 11614-1-AP
IP result of anti-RBM4 (IP:11614-1-AP, 3ug; Detection:11614-1-AP 1:1000) with mouse heart tissue lysate 4000ug.
IHC staining of human gliomas using 11614-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11614-1-AP (RBM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 11614-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11614-1-AP (RBM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 11614-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11614-1-AP (RBM4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 11614-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 11614-1-AP (RBM4 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human gliomas using 11614-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11614-1-AP (RBM4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

RBM4 Polyclonal antibody

RBM4 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 11614-1-AP

Publications(21)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, RIP, ELISA, PLA

Synonyms

hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite®594

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (21)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, human brain tissue, human heart tissue, human kidney tissue, HeLa cells
Positive IP detected in	mouse heart tissue
Positive IHC detected in	human gliomas tissue, human lung cancer tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 8 publications below
WB	See 18 publications below
IHC	See 7 publications below
IF	See 5 publications below
IP	See 1 publications below
FC	See 1 publications below
RIP	See 1 publications below

Product Information

11614-1-AP targets RBM4 in WB, IHC, IF, IP, RIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2190 Product name: Recombinant human RBM4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-177 aa of BC021120 Sequence: MVKLFIGNLPREATEQEIRSLFEQYGKVLECDIIKNYGFVHIEDKTAAEDAIRNLHHYKLHGVNINVEASKNKSKTSTKLHVGNISPTCTNKELRAKFEEYGPVIECDIVKDYAFVHMERAEDAVEAIRGLDNTEFQGEPPSLGRGLNTRLCAENGWISKRRGLVKITAVGWLVMKK 相同性解析による交差性が予測される生物種
Full Name	RNA binding motif protein 4
Calculated molecular weight	40 kDa
Observed molecular weight	40 kDa
GenBank accession number	BC021120
Gene Symbol	RBM4
Gene ID (NCBI)	5936
RRID	AB_2176095
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BWF3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis. This is a rabbit polyclonal antibody raised against the full-length of human RBM4

Protocols

Product Specific Protocols
IHC protocol for RBM4 antibody 11614-1-AP	Download protocol
IP protocol for RBM4 antibody 11614-1-AP	Download protocol
WB protocol for RBM4 antibody 11614-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nature An oxygen-regulated switch in the protein synthesis machinery. Authors - Uniacke James J View Article
human	WB, IHC	Cancer Cell The splicing factor RBM4 controls apoptosis, proliferation, and migration to suppress tumor progression. Authors - Yang Wang KD Validated View Article
human	WB	Nucleic Acids Res Modulation of alternative splicing during early infection of human primary B lymphocytes with Epstein-Barr virus (EBV): a novel function for the viral EBNA-LP protein. Authors - Evelyne Manet View Article
human	WB	Nat Commun A splicing isoform of TEAD4 attenuates the Hippo-YAP signalling to inhibit tumour proliferation. Authors - Yangfan Qi View Article
human	WB	Int J Mol Sci G-Quadruplex Regulation of VEGFA mRNA Translation by RBM4. Authors - Kangkang Niu KD Validated View Article
human	IHC	Sci Rep Prognostic value of decreased expression of RBM4 in human gastric cancer. Authors - Hongmei Yong View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa using 11614-1-AP

WB analysis of mouse brain using 11614-1-AP

WB analysis of human heart using 11614-1-AP

WB analysis of human brain using 11614-1-AP

WB analysis of mouse brain using 11614-1-AP

WB analysis of human heart using 11614-1-AP

WB analysis of human brain using 11614-1-AP

WB analysis of mouse brain using 11614-1-AP

WB analysis of human kidney using 11614-1-AP

IP experiment of mouse heart using 11614-1-AP

IHC staining of human gliomas using 11614-1-AP

IHC staining of human stomach cancer using 11614-1-AP

IHC staining of human stomach cancer using 11614-1-AP

IHC staining of human lung cancer using 11614-1-AP

IHC staining of human gliomas using 11614-1-AP

RBM4 Polyclonal antibody

RBM4 Polyclonal Antibody for WB, IP, IHC, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

An oxygen-regulated switch in the protein synthesis machinery.

The splicing factor RBM4 controls apoptosis, proliferation, and migration to suppress tumor progression.

Modulation of alternative splicing during early infection of human primary B lymphocytes with Epstein-Barr virus (EBV): a novel function for the viral EBNA-LP protein.

A splicing isoform of TEAD4 attenuates the Hippo-YAP signalling to inhibit tumour proliferation.

G-Quadruplex Regulation of VEGFA mRNA Translation by RBM4.

Prognostic value of decreased expression of RBM4 in human gastric cancer.