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  • KD/KO Validated

RBM4 Polyclonal antibody

RBM4 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, RIP, PLA, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11614-1-AP

Synonyms

hLark, LARK, Lark homolog, RBM4, RBM4A, RNA binding motif protein 4, RNA binding motif protein 4a, RNA binding protein 4, ZCCHC21, ZCRB3A



Tested Applications

Positive WB detected inmouse brain tissue, human brain tissue, human heart tissue, human kidney tissue, HeLa cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman gliomas tissue, human lung cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11614-1-AP targets RBM4 in WB, RIP, PLA, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM4 fusion protein Ag2190 相同性解析による交差性が予測される生物種
Full Name RNA binding motif protein 4
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC021120
Gene symbol RBM4
Gene ID (NCBI) 5936
RRIDAB_2176095
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

RBM4, RNA-binding motif protein4, contains 2 RRM-type RNA-binding motif and a retroviral-type(RT) zinc finger. RNA-binding factor participates in number of aspects of cellular processes such as alternative splicing of pre-mRNA and translation regulation. RBM4 recruits eIF4A1 to stimulate IRES-dependent translation in responds to cellular stress. Once assembled at the rHRE, the HIF2A-RBM4-eIF4E2 complex captures the 5-prime cap and targets mRNAs to polysomes for active translation, thereby evading hypoxia-induced repression of protein synthesis. This is a rabbit polyclonal antibody raised against the full-length of human RBM4

Protocols

Product Specific Protocols
WB protocol for RBM4 antibody 11614-1-APDownload protocol
IHC protocol for RBM4 antibody 11614-1-APDownload protocol
IP protocol for RBM4 antibody 11614-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

An oxygen-regulated switch in the protein synthesis machinery.

Authors - Uniacke James J
humanWB, IHC

Cancer Cell

The splicing factor RBM4 controls apoptosis, proliferation, and migration to suppress tumor progression.

Authors - Yang Wang
  • KD Validated
humanWB

Nucleic Acids Res

Modulation of alternative splicing during early infection of human primary B lymphocytes with Epstein-Barr virus (EBV): a novel function for the viral EBNA-LP protein.

Authors - Evelyne Manet
humanWB

Nat Commun

A splicing isoform of TEAD4 attenuates the Hippo-YAP signalling to inhibit tumour proliferation.

Authors - Yangfan Qi
humanWB

Int J Mol Sci

G-Quadruplex Regulation of VEGFA mRNA Translation by RBM4.

Authors - Kangkang Niu
  • KD Validated
humanIHC

Sci Rep

Prognostic value of decreased expression of RBM4 in human gastric cancer.

Authors - Hongmei Yong