Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse testis tissue, A375 cells, human lung tissue, human spleen tissue, mouse lung tissue |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | BxPC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
Product Information
12230-2-AP targets RBBP9 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2868 Product name: Recombinant human RBBP9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-186 aa of BC015938 Sequence: MASPSKAVIVPGNGGGDVTTHGWYGWVKKELEKIPGFQCLAKNMPDPITARESIWLPFMETELHCDEKTIIIGHSSGAIAAMRYAETHRVYAIVLVSAYTSDLGDENERASGYFTRPWQWEKIKANCPYIVQFGSTDDPFLPWKEQQEVADRLETKLHKFTDCGHFQNTEFHELITVVKSLLKVPA 相同性解析による交差性が予測される生物種 |
| Full Name | retinoblastoma binding protein 9 |
| Calculated molecular weight | 186 aa, 21 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC015938 |
| Gene Symbol | RBBP9 |
| Gene ID (NCBI) | 10741 |
| RRID | AB_2175265 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75884 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
RBBP9, also named as BOG, RBBP10, RBBP-9, RBBP-10 and Protein BOG, belongs to the RBBP9 family. It may play a role in the transformation process due to its capacity to confer resistance to the growth-inhibitory effects of TGF-β1 through interaction with retinoblastoma and the subsequent displacement of E2F-1. RBBP9 is a tumor-associated serine hydrolase activity required for pancreatic neoplasia. It mediates suppression of TGF-β signaling is required for E-cadherin expression as loss of the serine hydrolase activity leads to a reduction in E-cadherin levels and a concomitant decrease in the integrity of tumor cell-cell junctions..RBBP9 protein levels were equivalent in paired primary tumor and nonneoplastic specimens(PMID: 20080647) The genes CyFIP2 and RbBP9, which are also miss-expressed in ALS hMSC, could serve as diagnostic biomarker tools for detection of ALS in blood samples.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for RBBP9 antibody 12230-2-AP | Download protocol |
| IHC protocol for RBBP9 antibody 12230-2-AP | Download protocol |
| IP protocol for RBBP9 antibody 12230-2-AP | Download protocol |
| WB protocol for RBBP9 antibody 12230-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Proc Natl Acad Sci U S A RBBP9: a tumor-associated serine hydrolase activity required for pancreatic neoplasia. | ||
Cell Mol Gastroenterol Hepatol Retinoblastoma-Binding Protein 9 Suppresses Intestinal Inflammation and Inflammation-Induced Tumorigenesis in Mice
| ||
Antioxid Redox Signal Regulation of the 20S Proteasome by a Novel Family of Inhibitory Proteins. | ||
Exp Hematol Retinoblastoma-binding proteins 4 and 9 are important for human pluripotent stem cell maintenance.
| ||
Dis Markers Two potential biomarkers identified in mesenchymal stem cells and leukocytes of patients with sporadic amyotrophic lateral sclerosis. |
