RASGRP3 Monoclonal antibody
RASGRP3 Monoclonal Antibody for WB, IF, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
Human, mouse
Applications
WB, IF, ELISA
Conjugate
Unconjugated
CloneNo.
1A10G4
Cat no : 66650-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Raji cells, HEK-293 cells, Jurkat cells, Ramos cells, Raw 264.7 cells |
| Positive IF detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| Sample-dependent, check data in validation data gallery | |
Product Information
66650-1-Ig targets RASGRP3 in WB, IF, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | RASGRP3 fusion protein Ag3810 相同性解析による交差性が予測される生物種 |
| Full Name | RAS guanyl releasing protein 3 (calcium and DAG-regulated) |
| Calculated molecular weight | 690 aa, 78 kDa |
| Observed molecular weight | 78 kDa |
| GenBank accession number | BC027849 |
| Gene symbol | RASGRP3 |
| Gene ID (NCBI) | 25780 |
| RRID | AB_2882007 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
AS guanyl releasing protein 3 (RasGRP3) is a member of the RasGRP family and is able to promote guanine nucleotide exchange of Ha-Ras, R-Ras and Rap1 (PMID: 9582122). RasGRP3 is involved in a diverse range of important biological processes. RasGRP3 has emerged as an important mediator of signaling downstream from receptor coupled phosphoinositide turnover in B and T cells. Overexpression of RasGRP3 is commonly associated with many malignancies, such as pre-Bcell leukemia, Burkitt’s lymphoma and natural killer-like T-cell leukemia. Recently, it has been reported that upregulation of RASGRP3 expression in prostate cancer correlates with aggressive capabilities and predicts biochemical recurrence after radical prostatectomy (PMID: 24418912).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RASGRP3 antibody 66650-1-Ig | Download protocol |
| IF protocol for RASGRP3 antibody 66650-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Carly (Verified Customer) (09-27-2019) | Used in an antibody array to identify proteins in human EDTA plasma.
|
