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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

RASEF Monoclonal antibody

RASEF Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C2B2

Cat no : 67159-1-Ig

Synonyms

FLJ31614, RAB45, Ras related protein Rab 45, RASEF

Validation Data Gallery

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  • Western Blot (WB) analysis of LNCaP cells using RASEF Monoclonal antibody (67159-1-Ig)

    WB analysis of LNCaP using 67159-1-Ig

    LNCaP cells were subjected to SDS PAGE followed by western blot with 67159-1-Ig (RASEF antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of mouse pancreas using 67159-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human prostate cancer using 67159-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human prostate cancer using 67159-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of mouse pancreas using 67159-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human ovary tumor using 67159-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human ovary tumor using 67159-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human pancreas cancer using 67159-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using RASEF Monoclonal antibody (67159-1-Ig)

    IHC staining of human pancreas cancer using 67159-1-Ig

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67159-1-Ig (RASEF antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using RASEF Monoclonal antibody (67159-1-Ig)

    IF Staining of A431 using 67159-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RASEF antibody (67159-1-Ig, Clone: 1C2B2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inLNCaP cells
Positive IHC detected inhuman prostate cancer tissue, human ovary tumor tissue, human pancreas cancer tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67159-1-Ig targets RASEF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen RASEF fusion protein Ag2149 相同性解析による交差性が予測される生物種
Full Name RAS and EF-hand domain containing
Calculated molecular weight 740 aa, 83 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC023566
Gene symbol RASEF
Gene ID (NCBI) 158158
RRIDAB_2882456
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The RASEF (RAS and EF-hand domain-containing protein), a member of Rab GTPase protein family, contains a Rab GTPase domain in the C-terminal region, a coiled coil motif in an internal region as well as 2 EF hand domains which were of importance for binding to calcium ions in the N-terminus.

Protocols

Product Specific Protocols
WB protocol for RASEF antibody 67159-1-IgDownload protocol
IHC protocol for RASEF antibody 67159-1-IgDownload protocol
IF protocol for RASEF antibody 67159-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols